Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus

O Castañeda, V Sotolongo, A M Amor, R Stöcklin, A J Anderson, A L Harvey, A Engström, C Wernstedt, E Karlsson

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

A peptide toxin, ShK, that blocks voltage-dependent potassium channels was isolated from the whole body extract of the Caribbean sea anemone Stichodactyla helianthus. It competes with dendrotoxin I and alpha-dendrotoxin for binding to synaptosomal membranes of rat brain, facilities acetylcholine release at an avian neuromuscular junction and suppresses K+ currents in rat dorsal root ganglion neurones in culture. Its amino acid sequence is R1SCIDTIPKS10RCTAFQCKHS20MKYRLSFCRK30TCGTC35. There is no homology with other K+ channel-blocking peptides, except for BgK from the sea anemone Bunodosoma granulifera. ShK and BgK appear to be in a different structural class from other toxins affecting K+ channels.
LanguageEnglish
Pages603-613
Number of pages11
JournalToxicon
Volume33
Issue number5
DOIs
Publication statusPublished - May 1995

Fingerprint

Sea Anemones
Helianthus
Potassium Channels
Rats
Peptides
Neuromuscular Junction
Spinal Ganglia
Acetylcholine
Neurons
Amino Acid Sequence
Brain
Membranes
Amino Acids
Electric potential
ShK neurotoxin
dendrotoxin I
dendrotoxin

Keywords

  • amino acid sequence
  • amino acids
  • animals
  • cnidarian venoms
  • molecular sequence data
  • potassium channel blockers
  • potassium channels
  • rats
  • sea anemones
  • synaptosomes

Cite this

Castañeda, O ; Sotolongo, V ; Amor, A M ; Stöcklin, R ; Anderson, A J ; Harvey, A L ; Engström, A ; Wernstedt, C ; Karlsson, E. / Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus. In: Toxicon. 1995 ; Vol. 33, No. 5. pp. 603-613.
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Castañeda, O, Sotolongo, V, Amor, AM, Stöcklin, R, Anderson, AJ, Harvey, AL, Engström, A, Wernstedt, C & Karlsson, E 1995, 'Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus' Toxicon, vol. 33, no. 5, pp. 603-613. https://doi.org/10.1016/0041-0101(95)00013-C

Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus. / Castañeda, O; Sotolongo, V; Amor, A M; Stöcklin, R; Anderson, A J; Harvey, A L; Engström, A; Wernstedt, C; Karlsson, E.

In: Toxicon, Vol. 33, No. 5, 05.1995, p. 603-613.

Research output: Contribution to journalArticle

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T1 - Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus

AU - Castañeda, O

AU - Sotolongo, V

AU - Amor, A M

AU - Stöcklin, R

AU - Anderson, A J

AU - Harvey, A L

AU - Engström, A

AU - Wernstedt, C

AU - Karlsson, E

PY - 1995/5

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N2 - A peptide toxin, ShK, that blocks voltage-dependent potassium channels was isolated from the whole body extract of the Caribbean sea anemone Stichodactyla helianthus. It competes with dendrotoxin I and alpha-dendrotoxin for binding to synaptosomal membranes of rat brain, facilities acetylcholine release at an avian neuromuscular junction and suppresses K+ currents in rat dorsal root ganglion neurones in culture. Its amino acid sequence is R1SCIDTIPKS10RCTAFQCKHS20MKYRLSFCRK30TCGTC35. There is no homology with other K+ channel-blocking peptides, except for BgK from the sea anemone Bunodosoma granulifera. ShK and BgK appear to be in a different structural class from other toxins affecting K+ channels.

AB - A peptide toxin, ShK, that blocks voltage-dependent potassium channels was isolated from the whole body extract of the Caribbean sea anemone Stichodactyla helianthus. It competes with dendrotoxin I and alpha-dendrotoxin for binding to synaptosomal membranes of rat brain, facilities acetylcholine release at an avian neuromuscular junction and suppresses K+ currents in rat dorsal root ganglion neurones in culture. Its amino acid sequence is R1SCIDTIPKS10RCTAFQCKHS20MKYRLSFCRK30TCGTC35. There is no homology with other K+ channel-blocking peptides, except for BgK from the sea anemone Bunodosoma granulifera. ShK and BgK appear to be in a different structural class from other toxins affecting K+ channels.

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KW - cnidarian venoms

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KW - potassium channel blockers

KW - potassium channels

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