Characterisation of the antiproliferative constituents and activity of Ficus exasperata (Vahl) on ovarian cancer cells – a preliminary investigation

Enitome E. Bafor, Jennifer McKenna, Edward G. Rowan, RuAngelie Edrada-Ebel

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4 Citations (Scopus)
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Abstract

Ovarian cancer is one of the most common gynaecological cancers today. This study therefore investigates the anticancer effects of Ficus exasperata extracts and fractions on ovarian cancer cells. The antiproliferative activity of the crude extracts (1 mg/mL) was assessed using the MTT assay on A2780 (ovarian cancer) cell line. Bio-activity guided fractionation was performed and preliminary identification was further achieved using high resolution mass spectrometry and nuclear magnetic resonance spectroscopy. All crude extracts tested exhibited antiproliferative activity except for the methanol extract which interestingly showed proliferative effects. Five fatty acids were identified from the active fractions (FB1-10 and FB1-12). FB1-12 exhibited an IC50 value of 15.20 μg/mL. The least potent fraction (FB1-4 + 5) had an IC50 value of 34.51 μg/mL. H1-HEX and H1-MET exhibited 97.2 and 97.9%, respectively, compared to control. This study therefore provides proof-of-principle that fatty acids of Ficus exasperata exhibit significant antiproliferative effects on ovarian cancer cells.

Original languageEnglish
Pages (from-to)2164-2168
Number of pages5
JournalNatural Product Research
Volume31
Issue number18
Early online date17 Jan 2017
DOIs
Publication statusPublished - 1 Sep 2017

Keywords

  • A2780 cells
  • fatty acids
  • Ficus exasperata
  • flash chromatography
  • MTT assay
  • NMR
  • ovarian cancer

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