Characterisation of neural stem cells for oestrogen in vitro: potential for improving stem cell based therapy for stroke

S. Patkar, R. Tate, M. Modo, R.J. Plevin, H.V.O. Carswell

Research output: Contribution to journalConference Contribution

Abstract

This paper examines the characterisation of neural stem cells for oestrogen in vitro. Stroke is a problem for the ageing population. The ageing population is increasing but there is no licensed therapy for chronic stages of the disease. Stem cells are already known to have enormous potential to improve stroke outcome in the chronic stages1 but we need to improve their integration in vivo. Evidence also suggests that the female hormone, oestrogen, enhances differentiation of neural stem cells.2 The long-term aim of the present study is to determine whether oestrogen can improve the success of neural stem cell grafting in experimental stroke. We used an immortalised temperature sensitive murine neural stem cell line, the Maudsley hippocampal stem cell line clone 36 (MHP36) because they proliferate only at low temperatures (33°C) in vitro, develop into mature neurons and glia on transplantation into the higher temperature brain (37°C) and cease dividing once matured reducing the chance of producing tumours. The short-term aim was to fully characterise the MHP36 for oestrogen receptors (ER) and the enzyme, aromatase, which synthesizes oestrogen.
LanguageEnglish
PagesS554-S555
JournalJournal of Cerebral Blood Flow and Metabolism
Volume29
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Neural Stem Cells
Cell- and Tissue-Based Therapy
Estrogens
Stem Cells
Stroke
Temperature
Cell Line
Aromatase
Neuroglia
Estrogen Receptors
Population
Chronic Disease
Clone Cells
Transplantation
Hormones
Neurons
In Vitro Techniques
Brain
Enzymes
Neoplasms

Keywords

  • neural stem cells
  • oestrogen
  • vitro
  • stem cell
  • therapyl
  • stroke

Cite this

@article{a18f738164ea4b0e8f84448abcdc918d,
title = "Characterisation of neural stem cells for oestrogen in vitro: potential for improving stem cell based therapy for stroke",
abstract = "This paper examines the characterisation of neural stem cells for oestrogen in vitro. Stroke is a problem for the ageing population. The ageing population is increasing but there is no licensed therapy for chronic stages of the disease. Stem cells are already known to have enormous potential to improve stroke outcome in the chronic stages1 but we need to improve their integration in vivo. Evidence also suggests that the female hormone, oestrogen, enhances differentiation of neural stem cells.2 The long-term aim of the present study is to determine whether oestrogen can improve the success of neural stem cell grafting in experimental stroke. We used an immortalised temperature sensitive murine neural stem cell line, the Maudsley hippocampal stem cell line clone 36 (MHP36) because they proliferate only at low temperatures (33°C) in vitro, develop into mature neurons and glia on transplantation into the higher temperature brain (37°C) and cease dividing once matured reducing the chance of producing tumours. The short-term aim was to fully characterise the MHP36 for oestrogen receptors (ER) and the enzyme, aromatase, which synthesizes oestrogen.",
keywords = "neural stem cells, oestrogen, vitro, stem cell, therapyl, stroke",
author = "S. Patkar and R. Tate and M. Modo and R.J. Plevin and H.V.O. Carswell",
year = "2009",
month = "10",
doi = "10.1038/jcbfm.2009.168",
language = "English",
volume = "29",
pages = "S554--S555",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",

}

TY - JOUR

T1 - Characterisation of neural stem cells for oestrogen in vitro: potential for improving stem cell based therapy for stroke

AU - Patkar, S.

AU - Tate, R.

AU - Modo, M.

AU - Plevin, R.J.

AU - Carswell, H.V.O.

PY - 2009/10

Y1 - 2009/10

N2 - This paper examines the characterisation of neural stem cells for oestrogen in vitro. Stroke is a problem for the ageing population. The ageing population is increasing but there is no licensed therapy for chronic stages of the disease. Stem cells are already known to have enormous potential to improve stroke outcome in the chronic stages1 but we need to improve their integration in vivo. Evidence also suggests that the female hormone, oestrogen, enhances differentiation of neural stem cells.2 The long-term aim of the present study is to determine whether oestrogen can improve the success of neural stem cell grafting in experimental stroke. We used an immortalised temperature sensitive murine neural stem cell line, the Maudsley hippocampal stem cell line clone 36 (MHP36) because they proliferate only at low temperatures (33°C) in vitro, develop into mature neurons and glia on transplantation into the higher temperature brain (37°C) and cease dividing once matured reducing the chance of producing tumours. The short-term aim was to fully characterise the MHP36 for oestrogen receptors (ER) and the enzyme, aromatase, which synthesizes oestrogen.

AB - This paper examines the characterisation of neural stem cells for oestrogen in vitro. Stroke is a problem for the ageing population. The ageing population is increasing but there is no licensed therapy for chronic stages of the disease. Stem cells are already known to have enormous potential to improve stroke outcome in the chronic stages1 but we need to improve their integration in vivo. Evidence also suggests that the female hormone, oestrogen, enhances differentiation of neural stem cells.2 The long-term aim of the present study is to determine whether oestrogen can improve the success of neural stem cell grafting in experimental stroke. We used an immortalised temperature sensitive murine neural stem cell line, the Maudsley hippocampal stem cell line clone 36 (MHP36) because they proliferate only at low temperatures (33°C) in vitro, develop into mature neurons and glia on transplantation into the higher temperature brain (37°C) and cease dividing once matured reducing the chance of producing tumours. The short-term aim was to fully characterise the MHP36 for oestrogen receptors (ER) and the enzyme, aromatase, which synthesizes oestrogen.

KW - neural stem cells

KW - oestrogen

KW - vitro

KW - stem cell

KW - therapyl

KW - stroke

U2 - 10.1038/jcbfm.2009.168

DO - 10.1038/jcbfm.2009.168

M3 - Conference Contribution

VL - 29

SP - S554-S555

JO - Journal of Cerebral Blood Flow and Metabolism

T2 - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

ER -