Characterisation of natural carious lesions by flurescence spectroscopy at 405nm excitation wavelength

D.M. Zezell, A.C. Ribiero, L. Bachmann, A.S.L. Gomes, A.F. Hall, C. Rousseau, J.M. Girkin

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We aim to characterize natural caries enamel lesions by fluorescence spectroscopy. Sixty human samples with natural noncavitated caries lesions on smooth surfaces were selected and classified into three groups: dull, shiny, and brown lesions. All the samples were analyzed externally at the natural surface and after hemisectionig internally at the center of the lesion. The lesions were excited with a 405-nm InGaN diode laser and the fluorescence was collected with a single grating spectrometer. Four emission bands (455, 500, 582, and 622 nm) are identified in both sound and carious regions. The area under each emission band is correlated with the total area of the four bands for the sound and carious regions. The detected fluorescence from natural and cut surfaces through the caries lesions is not statistically different for the shiny and dull lesion, but is different [analysis of variance (ANOVA) (p<0.05)] for brown lesion at all emission bands. At the 405-nm excitation wavelength, the area of the fluorescence bands at 455 and 500 nm differ statistically for natural carious lesions and sound tissue.
Original languageEnglish
Article number064013
Pages (from-to)303
JournalJournal of Biomedical Optics
Volume12
DOIs
Publication statusPublished - 2005

Fingerprint

lesions
Fluorescence
Acoustic waves
Spectroscopy
Wavelength
wavelengths
spectroscopy
excitation
Enamels
Diffraction gratings
Fluorescence spectroscopy
Analysis of variance (ANOVA)
Semiconductor lasers
Spectrometers
fluorescence
Tissue
acoustics
analysis of variance
enamels
semiconductor lasers

Keywords

  • carious lesions
  • flurescence spectroscopy
  • excitation wavelength

Cite this

Zezell, D. M., Ribiero, A. C., Bachmann, L., Gomes, A. S. L., Hall, A. F., Rousseau, C., & Girkin, J. M. (2005). Characterisation of natural carious lesions by flurescence spectroscopy at 405nm excitation wavelength. Journal of Biomedical Optics, 12, 303. [064013]. https://doi.org/10.1117/1.2821192
Zezell, D.M. ; Ribiero, A.C. ; Bachmann, L. ; Gomes, A.S.L. ; Hall, A.F. ; Rousseau, C. ; Girkin, J.M. / Characterisation of natural carious lesions by flurescence spectroscopy at 405nm excitation wavelength. In: Journal of Biomedical Optics. 2005 ; Vol. 12. pp. 303.
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abstract = "We aim to characterize natural caries enamel lesions by fluorescence spectroscopy. Sixty human samples with natural noncavitated caries lesions on smooth surfaces were selected and classified into three groups: dull, shiny, and brown lesions. All the samples were analyzed externally at the natural surface and after hemisectionig internally at the center of the lesion. The lesions were excited with a 405-nm InGaN diode laser and the fluorescence was collected with a single grating spectrometer. Four emission bands (455, 500, 582, and 622 nm) are identified in both sound and carious regions. The area under each emission band is correlated with the total area of the four bands for the sound and carious regions. The detected fluorescence from natural and cut surfaces through the caries lesions is not statistically different for the shiny and dull lesion, but is different [analysis of variance (ANOVA) (p<0.05)] for brown lesion at all emission bands. At the 405-nm excitation wavelength, the area of the fluorescence bands at 455 and 500 nm differ statistically for natural carious lesions and sound tissue.",
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Zezell, DM, Ribiero, AC, Bachmann, L, Gomes, ASL, Hall, AF, Rousseau, C & Girkin, JM 2005, 'Characterisation of natural carious lesions by flurescence spectroscopy at 405nm excitation wavelength', Journal of Biomedical Optics, vol. 12, 064013, pp. 303. https://doi.org/10.1117/1.2821192

Characterisation of natural carious lesions by flurescence spectroscopy at 405nm excitation wavelength. / Zezell, D.M.; Ribiero, A.C.; Bachmann, L.; Gomes, A.S.L.; Hall, A.F.; Rousseau, C.; Girkin, J.M.

In: Journal of Biomedical Optics, Vol. 12, 064013, 2005, p. 303.

Research output: Contribution to journalArticle

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AU - Zezell, D.M.

AU - Ribiero, A.C.

AU - Bachmann, L.

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AU - Hall, A.F.

AU - Rousseau, C.

AU - Girkin, J.M.

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AB - We aim to characterize natural caries enamel lesions by fluorescence spectroscopy. Sixty human samples with natural noncavitated caries lesions on smooth surfaces were selected and classified into three groups: dull, shiny, and brown lesions. All the samples were analyzed externally at the natural surface and after hemisectionig internally at the center of the lesion. The lesions were excited with a 405-nm InGaN diode laser and the fluorescence was collected with a single grating spectrometer. Four emission bands (455, 500, 582, and 622 nm) are identified in both sound and carious regions. The area under each emission band is correlated with the total area of the four bands for the sound and carious regions. The detected fluorescence from natural and cut surfaces through the caries lesions is not statistically different for the shiny and dull lesion, but is different [analysis of variance (ANOVA) (p<0.05)] for brown lesion at all emission bands. At the 405-nm excitation wavelength, the area of the fluorescence bands at 455 and 500 nm differ statistically for natural carious lesions and sound tissue.

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