Characterisation of a novel mouse liver aldo-keto reductase AKR7A5

A. Hinshelwood, G. McGarvie, E. Ellis

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We have characterised a novel aldo-keto reductase (AKR7A5) from mouse liver that is 78% identical to rat aflatoxin dialdehyde reductase AKR7A1 and 89% identical to human succinic semialdehyde (SSA) reductase AKR7A2. AKR7A5 can reduce 2-carboxybenzaldehyde (2-CBA) and SSA as well as a range of aldehyde and diketone substrates. Western blots show that it is expressed in liver, kidney, testis and brain, and at lower levels in skeletal muscle, spleen heart and lung. The protein is not inducible in the liver by dietary ethoxyquin. Immunodepletion of AKR7A5 from liver extracts shows that it is one of the major liver 2-CBA reductases but that it is not the main SSA reductase in this tissue.
LanguageEnglish
Pages213-218
Number of pages6
JournalFEBS Letters
Volume523
Issue number1-3
DOIs
Publication statusPublished - 2002

Fingerprint

Liver
Ethoxyquin
Liver Extracts
Aflatoxins
Aldehydes
Muscle
Testis
Rats
Brain
Oxidoreductases
Skeletal Muscle
Spleen
Western Blotting
Tissue
Kidney
Lung
carbonyl reductase (NADPH)
Substrates
Proteins
4-hydroxybutyrate dehydrogenase

Keywords

  • aldo-keto reductase
  • carbonyl metabolism
  • succinic semialdehyde
  • mouse liver

Cite this

Hinshelwood, A. ; McGarvie, G. ; Ellis, E. / Characterisation of a novel mouse liver aldo-keto reductase AKR7A5. In: FEBS Letters. 2002 ; Vol. 523, No. 1-3. pp. 213-218.
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Hinshelwood, A, McGarvie, G & Ellis, E 2002, 'Characterisation of a novel mouse liver aldo-keto reductase AKR7A5' FEBS Letters, vol. 523, no. 1-3, pp. 213-218. https://doi.org/10.1016/S0014-5793(02)02982-4

Characterisation of a novel mouse liver aldo-keto reductase AKR7A5. / Hinshelwood, A.; McGarvie, G.; Ellis, E.

In: FEBS Letters, Vol. 523, No. 1-3, 2002, p. 213-218.

Research output: Contribution to journalArticle

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T1 - Characterisation of a novel mouse liver aldo-keto reductase AKR7A5

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AU - McGarvie, G.

AU - Ellis, E.

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AB - We have characterised a novel aldo-keto reductase (AKR7A5) from mouse liver that is 78% identical to rat aflatoxin dialdehyde reductase AKR7A1 and 89% identical to human succinic semialdehyde (SSA) reductase AKR7A2. AKR7A5 can reduce 2-carboxybenzaldehyde (2-CBA) and SSA as well as a range of aldehyde and diketone substrates. Western blots show that it is expressed in liver, kidney, testis and brain, and at lower levels in skeletal muscle, spleen heart and lung. The protein is not inducible in the liver by dietary ethoxyquin. Immunodepletion of AKR7A5 from liver extracts shows that it is one of the major liver 2-CBA reductases but that it is not the main SSA reductase in this tissue.

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KW - carbonyl metabolism

KW - succinic semialdehyde

KW - mouse liver

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