Abstract
The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.
| Original language | English |
|---|---|
| Article number | 1600046 |
| Number of pages | 10 |
| Journal | Advanced Science |
| Volume | 3 |
| Issue number | 10 |
| Early online date | 27 May 2016 |
| DOIs | |
| Publication status | Published - 1 Oct 2016 |
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Keywords
- drug delivery nanosystems
- nucleotide delivery vehicle
- protein cages
- protein–polymer conjugates
- RNA interference
- small interfering RNA
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Chaperonin–dendrimer conjugates for siRNA delivery. / Nussbaumer, Martin G.; Duskey, Jason T.; Rother, Martin; Renggli, Kasper; Chami, Mohamed; Bruns, Nico.
In: Advanced Science, Vol. 3, No. 10, 1600046, 01.10.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Chaperonin–dendrimer conjugates for siRNA delivery
AU - Nussbaumer, Martin G.
AU - Duskey, Jason T.
AU - Rother, Martin
AU - Renggli, Kasper
AU - Chami, Mohamed
AU - Bruns, Nico
PY - 2016/10/1
Y1 - 2016/10/1
N2 - The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.
AB - The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.
KW - drug delivery nanosystems
KW - nucleotide delivery vehicle
KW - protein cages
KW - protein–polymer conjugates
KW - RNA interference
KW - small interfering RNA
UR - http://www.scopus.com/inward/record.url?scp=84991354998&partnerID=8YFLogxK
U2 - 10.1002/advs.201600046
DO - 10.1002/advs.201600046
M3 - Article
VL - 3
JO - Advanced Science
JF - Advanced Science
SN - 2198-3844
IS - 10
M1 - 1600046
ER -