Chaperonin–dendrimer conjugates for siRNA delivery

Martin G. Nussbaumer, Jason T. Duskey, Martin Rother, Kasper Renggli, Mohamed Chami, Nico Bruns

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

LanguageEnglish
Article number1600046
Number of pages10
JournalAdvanced Science
Volume3
Issue number10
Early online date27 May 2016
DOIs
Publication statusPublished - 1 Oct 2016

Fingerprint

Thermosomes
Chaperonins
Dendrimers
dendrimers
RNA
Small Interfering RNA
delivery
proteins
Proteins
Group II Chaperonins
Cell-Penetrating Peptides
Pancreatic Ribonuclease
RNA Stability
RNA Interference
Anchors
cells
Drug delivery
payloads
Nanoparticles
Peptides

Keywords

  • drug delivery nanosystems
  • nucleotide delivery vehicle
  • protein cages
  • protein–polymer conjugates
  • RNA interference
  • small interfering RNA

Cite this

Nussbaumer, M. G., Duskey, J. T., Rother, M., Renggli, K., Chami, M., & Bruns, N. (2016). Chaperonin–dendrimer conjugates for siRNA delivery. Advanced Science, 3(10), [1600046]. https://doi.org/10.1002/advs.201600046
Nussbaumer, Martin G. ; Duskey, Jason T. ; Rother, Martin ; Renggli, Kasper ; Chami, Mohamed ; Bruns, Nico. / Chaperonin–dendrimer conjugates for siRNA delivery. In: Advanced Science. 2016 ; Vol. 3, No. 10.
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Nussbaumer, MG, Duskey, JT, Rother, M, Renggli, K, Chami, M & Bruns, N 2016, 'Chaperonin–dendrimer conjugates for siRNA delivery' Advanced Science, vol. 3, no. 10, 1600046. https://doi.org/10.1002/advs.201600046

Chaperonin–dendrimer conjugates for siRNA delivery. / Nussbaumer, Martin G.; Duskey, Jason T.; Rother, Martin; Renggli, Kasper; Chami, Mohamed; Bruns, Nico.

In: Advanced Science, Vol. 3, No. 10, 1600046, 01.10.2016.

Research output: Contribution to journalArticle

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Nussbaumer MG, Duskey JT, Rother M, Renggli K, Chami M, Bruns N. Chaperonin–dendrimer conjugates for siRNA delivery. Advanced Science. 2016 Oct 1;3(10). 1600046. https://doi.org/10.1002/advs.201600046