Chaperonin–dendrimer conjugates for siRNA delivery

Martin G. Nussbaumer; Jason T. Duskey; Martin Rother; Kasper Renggli; Mohamed Chami; Nico Bruns

doi:10.1002/advs.201600046

Chaperonin–dendrimer conjugates for siRNA delivery

Martin G. Nussbaumer, Jason T. Duskey, Martin Rother, Kasper Renggli, Mohamed Chami, Nico Bruns

Pure And Applied Chemistry

Research output: Contribution to journal › Article

21 Citations (Scopus)

8 Downloads (Pure)

Abstract

The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

Original language	English
Article number	1600046
Number of pages	10
Journal	Advanced Science
Volume	3
Issue number	10
Early online date	27 May 2016
DOIs	https://doi.org/10.1002/advs.201600046
Publication status	Published - 1 Oct 2016

Keywords

drug delivery nanosystems
nucleotide delivery vehicle
protein cages
protein–polymer conjugates
RNA interference
small interfering RNA

Access to Document

10.1002/advs.201600046Licence: CC BY 4.0

Nussbaumer-etal-AS-2016-Chaperonin-dendrimer-conjugates-for-siRNAFinal published version, 3.52 MBLicence: CC BY 4.0

Link to publication in Scopus

Fingerprint Dive into the research topics of 'Chaperonin–dendrimer conjugates for siRNA delivery'. Together they form a unique fingerprint.

Cite this

@article{309dd6d109114b18bb684f09019b61a6,

title = "Chaperonin–dendrimer conjugates for siRNA delivery",

abstract = "The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.",

keywords = "drug delivery nanosystems, nucleotide delivery vehicle, protein cages, protein–polymer conjugates, RNA interference, small interfering RNA",

author = "Nussbaumer, {Martin G.} and Duskey, {Jason T.} and Martin Rother and Kasper Renggli and Mohamed Chami and Nico Bruns",

year = "2016",

month = oct,

day = "1",

doi = "10.1002/advs.201600046",

language = "English",

volume = "3",

journal = "Advanced Science",

issn = "2198-3844",

number = "10",

}

TY - JOUR

T1 - Chaperonin–dendrimer conjugates for siRNA delivery

AU - Nussbaumer, Martin G.

AU - Duskey, Jason T.

AU - Rother, Martin

AU - Renggli, Kasper

AU - Chami, Mohamed

AU - Bruns, Nico

PY - 2016/10/1

Y1 - 2016/10/1

N2 - The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

AB - The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

KW - drug delivery nanosystems

KW - nucleotide delivery vehicle

KW - protein cages

KW - protein–polymer conjugates

KW - RNA interference

KW - small interfering RNA

UR - http://www.scopus.com/inward/record.url?scp=84991354998&partnerID=8YFLogxK

U2 - 10.1002/advs.201600046

DO - 10.1002/advs.201600046

M3 - Article

AN - SCOPUS:84991354998

VL - 3

JO - Advanced Science

JF - Advanced Science

SN - 2198-3844

IS - 10

M1 - 1600046

ER -