Chaperonin–dendrimer conjugates for siRNA delivery

Martin G. Nussbaumer, Jason T. Duskey, Martin Rother, Kasper Renggli, Mohamed Chami, Nico Bruns

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Abstract

The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

Original languageEnglish
Article number1600046
Number of pages10
JournalAdvanced Science
Volume3
Issue number10
Early online date27 May 2016
DOIs
Publication statusPublished - 1 Oct 2016

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Keywords

  • drug delivery nanosystems
  • nucleotide delivery vehicle
  • protein cages
  • protein–polymer conjugates
  • RNA interference
  • small interfering RNA

Cite this

Nussbaumer, M. G., Duskey, J. T., Rother, M., Renggli, K., Chami, M., & Bruns, N. (2016). Chaperonin–dendrimer conjugates for siRNA delivery. Advanced Science, 3(10), [1600046]. https://doi.org/10.1002/advs.201600046