Chaperonin–dendrimer conjugates for siRNA delivery

Martin G. Nussbaumer; Jason T. Duskey; Martin Rother; Kasper Renggli; Mohamed Chami; Nico Bruns

doi:10.1002/advs.201600046

Chaperonin–dendrimer conjugates for siRNA delivery

Martin G. Nussbaumer, Jason T. Duskey, Martin Rother, Kasper Renggli, Mohamed Chami, Nico Bruns

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

9 Downloads (Pure)

Abstract

The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.

Original language	English
Article number	1600046
Number of pages	10
Journal	Advanced Science
Volume	3
Issue number	10
Early online date	27 May 2016
DOIs	https://doi.org/10.1002/advs.201600046
Publication status	Published - 1 Oct 2016

Keywords

drug delivery nanosystems
nucleotide delivery vehicle
protein cages
protein–polymer conjugates
RNA interference
small interfering RNA

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title = "Chaperonin–dendrimer conjugates for siRNA delivery",

abstract = "The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.",

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AU - Duskey, Jason T.

AU - Rother, Martin

AU - Renggli, Kasper

AU - Chami, Mohamed

AU - Bruns, Nico

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