Changes in body posture alter plasma nitrite but not nitrate concentration in humans

Luke Liddle, Christopher Monaghan, Mia C. Burleigh, Luke C. McIlvenna, David J. Muggeridge, Chris Easton

Research output: Contribution to journalArticle

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Abstract

Purpose: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]
Methods: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ∼31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method.
Results: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P < 0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P < 0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P < 0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P = 0.02). Plasma [NO3-] was not different between measurement points in either condition (P > 0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05.
Conclusions: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.
LanguageEnglish
Pages59-65
Number of pages7
JournalNitric Oxide: Biology and Chemistry
Volume72
Early online date2 Dec 2017
DOIs
StatePublished - 30 Jan 2018

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Nitrites
Posture
Nitrates
Plasmas
Plasma Volume
Anethum graveolens
Luminescence
Chemiluminescence
Healthy Volunteers
Gases
Research Personnel
Exercise

Keywords

  • beetroot juice
  • exercise
  • plasma volume

Cite this

Liddle, L., Monaghan, C., Burleigh, M. C., McIlvenna, L. C., Muggeridge, D. J., & Easton, C. (2018). Changes in body posture alter plasma nitrite but not nitrate concentration in humans. Nitric Oxide: Biology and Chemistry , 72, 59-65. DOI: 10.1016/j.niox.2017.11.008
Liddle, Luke ; Monaghan, Christopher ; Burleigh, Mia C. ; McIlvenna, Luke C. ; Muggeridge, David J. ; Easton, Chris. / Changes in body posture alter plasma nitrite but not nitrate concentration in humans. In: Nitric Oxide: Biology and Chemistry . 2018 ; Vol. 72. pp. 59-65
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abstract = "Purpose: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]Methods: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ∼31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method.Results: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P < 0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P < 0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P < 0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P = 0.02). Plasma [NO3-] was not different between measurement points in either condition (P > 0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05.Conclusions: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.",
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Liddle, L, Monaghan, C, Burleigh, MC, McIlvenna, LC, Muggeridge, DJ & Easton, C 2018, 'Changes in body posture alter plasma nitrite but not nitrate concentration in humans' Nitric Oxide: Biology and Chemistry , vol. 72, pp. 59-65. DOI: 10.1016/j.niox.2017.11.008

Changes in body posture alter plasma nitrite but not nitrate concentration in humans. / Liddle, Luke; Monaghan, Christopher; Burleigh, Mia C.; McIlvenna, Luke C.; Muggeridge, David J.; Easton, Chris.

In: Nitric Oxide: Biology and Chemistry , Vol. 72, 30.01.2018, p. 59-65.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Changes in body posture alter plasma nitrite but not nitrate concentration in humans

AU - Liddle,Luke

AU - Monaghan,Christopher

AU - Burleigh,Mia C.

AU - McIlvenna,Luke C.

AU - Muggeridge,David J.

AU - Easton,Chris

N1 - Copyright © 2017. Published by Elsevier Inc.

PY - 2018/1/30

Y1 - 2018/1/30

N2 - Purpose: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]Methods: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ∼31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method.Results: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P < 0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P < 0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P < 0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P = 0.02). Plasma [NO3-] was not different between measurement points in either condition (P > 0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05.Conclusions: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.

AB - Purpose: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]Methods: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ∼31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method.Results: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P < 0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P < 0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P < 0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P = 0.02). Plasma [NO3-] was not different between measurement points in either condition (P > 0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05.Conclusions: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.

KW - beetroot juice

KW - exercise

KW - plasma volume

UR - http://www.sciencedirect.com/science/journal/10898603

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DO - 10.1016/j.niox.2017.11.008

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SP - 59

EP - 65

JO - Nitric Oxide: Biology and Chemistry

T2 - Nitric Oxide: Biology and Chemistry

JF - Nitric Oxide: Biology and Chemistry

SN - 1089-8603

ER -

Liddle L, Monaghan C, Burleigh MC, McIlvenna LC, Muggeridge DJ, Easton C. Changes in body posture alter plasma nitrite but not nitrate concentration in humans. Nitric Oxide: Biology and Chemistry . 2018 Jan 30;72:59-65. Available from, DOI: 10.1016/j.niox.2017.11.008