Cellular uptake of gold nanoparticles triggered by Host-Guest interactions

Jesús Mosquera; Malou Henriksen-Lacey; Isabel García; Miguel Martínez-Calvo; Jéssica Rodríguez; José L. Mascareñas; Luis M. Liz-Marzán

doi:10.1021/jacs.7b12505

Cellular uptake of gold nanoparticles triggered by Host-Guest interactions

Jesús Mosquera^*, Malou Henriksen-Lacey, Isabel García, Miguel Martínez-Calvo, Jéssica Rodríguez, José L. Mascareñas, Luis M. Liz-Marzán

^*Corresponding author for this work

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

We describe an approach to regulate the cellular uptake of small gold nanoparticles using supramolecular chemistry. The strategy relies on the functionalization of AuNPs with negatively charged pyranines, which largely hamper their penetration in cells. Cellular uptake can be activated in situ through the addition of cationic covalent cages that specifically recognize the fluorescent pyranine dyes and counterbalance the negative charges. The high selectivity and reversibility of the host-guest recognition activates cellular uptake, even in protein-rich biological media, as well as its regulation by rational addition of either cage or pyranine.

Original language	English
Pages (from-to)	4469-4472
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	140
Issue number	13
Early online date	21 Mar 2018
DOIs	https://doi.org/10.1021/jacs.7b12505
Publication status	Published - 4 Apr 2018

Funding

This work has been funded by MINECO (Grant MAT2017-86659-R and SAF2016-76689-R), the Xunta de Galicia (2015-CP082, ED431C 2017/19 and Centro singular de investigacion de Galicia accreditation 2016-2019, ED431G/09), the European Regional Development Fund and the European Research Council (Advanced Grant No. 340055). J.M. and M.M.-C. thank MINECO for Juan de la Cierva-fellowships (FJCI-2015-25080 and IJCI-2014-19326). The authors thank Prof. Jonathan R. Nitschke for his assistance in the synthesis of A.

Keywords

cell uptake
metal nanoparticles
fluorescence
nanopartIcles
surface charge

Access to Document

10.1021/jacs.7b12505

Cite this

@article{fb894c4da75841278f1ffd72fc3e5cc8,

title = "Cellular uptake of gold nanoparticles triggered by Host-Guest interactions",

abstract = "We describe an approach to regulate the cellular uptake of small gold nanoparticles using supramolecular chemistry. The strategy relies on the functionalization of AuNPs with negatively charged pyranines, which largely hamper their penetration in cells. Cellular uptake can be activated in situ through the addition of cationic covalent cages that specifically recognize the fluorescent pyranine dyes and counterbalance the negative charges. The high selectivity and reversibility of the host-guest recognition activates cellular uptake, even in protein-rich biological media, as well as its regulation by rational addition of either cage or pyranine.",

keywords = "cell uptake, metal nanoparticles, fluorescence, nanopartIcles, surface charge",

author = "Jes{\'u}s Mosquera and Malou Henriksen-Lacey and Isabel Garc{\'i}a and Miguel Mart{\'i}nez-Calvo and J{\'e}ssica Rodr{\'i}guez and Mascare{\~n}as, \{Jos{\'e} L.\} and Liz-Marz{\'a}n, \{Luis M.\}",

year = "2018",

month = apr,

day = "4",

doi = "10.1021/jacs.7b12505",

language = "English",

volume = "140",

pages = "4469--4472",

journal = "Journal of the American Chemical Society ",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "13",

}

TY - JOUR

T1 - Cellular uptake of gold nanoparticles triggered by Host-Guest interactions

AU - Mosquera, Jesús

AU - Henriksen-Lacey, Malou

AU - García, Isabel

AU - Martínez-Calvo, Miguel

AU - Rodríguez, Jéssica

AU - Mascareñas, José L.

AU - Liz-Marzán, Luis M.

PY - 2018/4/4

Y1 - 2018/4/4

N2 - We describe an approach to regulate the cellular uptake of small gold nanoparticles using supramolecular chemistry. The strategy relies on the functionalization of AuNPs with negatively charged pyranines, which largely hamper their penetration in cells. Cellular uptake can be activated in situ through the addition of cationic covalent cages that specifically recognize the fluorescent pyranine dyes and counterbalance the negative charges. The high selectivity and reversibility of the host-guest recognition activates cellular uptake, even in protein-rich biological media, as well as its regulation by rational addition of either cage or pyranine.

AB - We describe an approach to regulate the cellular uptake of small gold nanoparticles using supramolecular chemistry. The strategy relies on the functionalization of AuNPs with negatively charged pyranines, which largely hamper their penetration in cells. Cellular uptake can be activated in situ through the addition of cationic covalent cages that specifically recognize the fluorescent pyranine dyes and counterbalance the negative charges. The high selectivity and reversibility of the host-guest recognition activates cellular uptake, even in protein-rich biological media, as well as its regulation by rational addition of either cage or pyranine.

KW - cell uptake

KW - metal nanoparticles

KW - fluorescence

KW - nanopartIcles

KW - surface charge

UR - https://www.scopus.com/pages/publications/85044964047

U2 - 10.1021/jacs.7b12505

DO - 10.1021/jacs.7b12505

M3 - Article

C2 - 29562135

AN - SCOPUS:85044964047

SN - 0002-7863

VL - 140

SP - 4469

EP - 4472

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 13

ER -

Cellular uptake of gold nanoparticles triggered by Host-Guest interactions

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this