Abstract
Transmission electron microscopy (TEM) studies revealed that rough cell-forms of L. monocytogenes (designated FR variants), isolated from clinical and food samples (and under conditions of sublethal heat stress), consist of either single or paired long-filaments. These FR variants markedly contrast in cell morphology from other previously described avirulent rough-mutants of L. monocytogenes that form long chains consisting of multiple cells of similar size (designated MCR variants). The identity of these Listeria isolates was determined using a commercially available, anti-Listeria polyclonal KPL antibody and by the API Listeria biochemical gallery. This study shows that filamentous rough-forms of L. monocytogenes may occur in clinical and food samples that are of undetermined pathogenicity.
| Original language | English |
|---|---|
| Pages (from-to) | 319-322 |
| Number of pages | 4 |
| Journal | Letters in Applied Microbiology |
| Volume | 31 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 31 Oct 2000 |
Keywords
- posibacteria
- bacterial mutation
- bacterium isolation
- bacterium mutant
- food analysis
- Listeria monocytogenes
- nonhuman
- pathogenicity
- transmission electron microscopy
- ultrastructure
- enzyme-linked immunosorbent assay
- food microbiology
- humans
- Listeria infections
- microscopy, electron
- bacteria (microorganisms)