Cellular interactions of surface modified nanoporous silicon particles

Luis M. Bimbo, Mirkka Sarparanta, Ermei Mäkilä, Timo Laaksonen, Päivi Laaksonen, Jarno Salonen, Markus B. Linder, Jouni Hirvonen, Anu J. Airaksinen, Hélder A. Santos

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46 Citations (Scopus)

Abstract

In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, (125)I-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.

Original languageEnglish
Pages (from-to)3184-3192
Number of pages9
JournalNanoscale
Volume4
Issue number10
Early online date16 Mar 2012
DOIs
Publication statusPublished - 21 May 2012
Externally publishedYes

Keywords

  • Caco-2 cells
  • cell line, tumor
  • cell survival
  • drug carriers
  • flow cytometry
  • humans
  • microscopy, confocal
  • nanoparticles
  • porosity
  • silicon
  • surface properties

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  • Cite this

    Bimbo, L. M., Sarparanta, M., Mäkilä, E., Laaksonen, T., Laaksonen, P., Salonen, J., Linder, M. B., Hirvonen, J., Airaksinen, A. J., & Santos, H. A. (2012). Cellular interactions of surface modified nanoporous silicon particles. Nanoscale, 4(10), 3184-3192. https://doi.org/10.1039/c2nr30397c