Cell adhesion and fluid flow jointly initiate genotype spatial distribution in biofilms

Ricardo Martínez-García, Carey D. Nadell, Raimo Hartmann, Knut Drescher, Juan A. Bonachela

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Biofilms are microbial collectives that occupy a diverse array of surfaces. It is well known that the function and evolution of biofilms are strongly influenced by the spatial arrangement of different strains and species within them, but how spatiotemporal distributions of different genotypes in biofilm populations originate is still underexplored. Here, we study the origins of biofilm genetic structure by combining model development, numerical simulations, and microfluidic experiments using the human pathogen Vibrio cholerae. Using spatial correlation functions to quantify the differences between emergent cell lineage segregation patterns, we find that strong adhesion often, but not always, maximizes the size of clonal cell clusters on flat surfaces. Counterintuitively, our model predicts that, under some conditions, investing in adhesion can reduce rather than increase clonal group size. Our results emphasize that a complex interaction between fluid flow and cell adhesiveness can underlie emergent patterns of biofilm genetic structure. This structure, in turn, has an outsize influence on how biofilm-dwelling populations function and evolve.

LanguageEnglish
Article numbere1006094
Number of pages19
JournalPLoS Computational Biology
Volume14
Issue number4
DOIs
Publication statusPublished - 16 Apr 2018

Fingerprint

Biofilm
Cell Adhesion
Cell adhesion
Biofilms
Genotype
adhesion
Spatial Distribution
cell adhesion
biofilm
Spatial distribution
Fluid Flow
fluid flow
Flow of fluids
genotype
spatial distribution
Genetic Structures
Adhesion
genetic structure
Cell
Adhesiveness

Keywords

  • planktonic cells
  • bacteria
  • biofilms
  • cell adhesion

Cite this

Martínez-García, R., Nadell, C. D., Hartmann, R., Drescher, K., & Bonachela, J. A. (2018). Cell adhesion and fluid flow jointly initiate genotype spatial distribution in biofilms. PLoS Computational Biology, 14(4), [e1006094]. https://doi.org/10.1371/journal.pcbi.1006094
Martínez-García, Ricardo ; Nadell, Carey D. ; Hartmann, Raimo ; Drescher, Knut ; Bonachela, Juan A. / Cell adhesion and fluid flow jointly initiate genotype spatial distribution in biofilms. In: PLoS Computational Biology. 2018 ; Vol. 14, No. 4.
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Martínez-García, R, Nadell, CD, Hartmann, R, Drescher, K & Bonachela, JA 2018, 'Cell adhesion and fluid flow jointly initiate genotype spatial distribution in biofilms' PLoS Computational Biology, vol. 14, no. 4, e1006094. https://doi.org/10.1371/journal.pcbi.1006094

Cell adhesion and fluid flow jointly initiate genotype spatial distribution in biofilms. / Martínez-García, Ricardo; Nadell, Carey D.; Hartmann, Raimo; Drescher, Knut; Bonachela, Juan A.

In: PLoS Computational Biology, Vol. 14, No. 4, e1006094, 16.04.2018.

Research output: Contribution to journalArticle

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