Cathodic quantum dot facilitated electrochemiluminescent detection

Alasdair J. Stewart, Kelly Brown, Lynn Dennany

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The expansion of electrochemical sensors to biomedical applications at point of care requires these sensors to undergo analysis without any pre-treatment of extraction. This poses a major challenge for all electrochemical sensors including electrochemiluminescent (ECL) based sensors. ECL offers many advantages for biomedical applications however; obtaining results from complex matrices has proven to be a large hurdle for the application of ECL sensors within this field. This work demonstrates the potential of cathodic ECL to detect and quantify homocysteine with 0.1 nM limit of detection, which is associated with hyperhomocysteinemia, in blood. This near infrared quantum dot (NIR QD) based ECL sensor displays good linearity allowing for rapid detection and providing a basis for exploitation of ECL based sensors for biomedical diagnostics utilising homocysteine as a model cathodic co-reactant. This work will lay the foundations for future developments in biosensing and imaging fields and stands as an initial proof of concept for the utilization of cathodic ECL technologies for biomedical applications once the limits of detection within clinically relevant levels has been achieved. This work illustrates the potential of cathodic ECL sensors, using Hcy as a model complex, for the detection of biomolecules.
LanguageEnglish
Pages12944-12950
Number of pages7
JournalAnalytical Chemistry
Volume90
Issue number21
Early online date3 Oct 2018
DOIs
Publication statusPublished - 6 Nov 2018

Fingerprint

Semiconductor quantum dots
Sensors
Electrochemical sensors
Homocysteine
Biomolecules
Blood
Infrared radiation
Imaging techniques

Keywords

  • electrochemical sensors
  • near infrared quantum dots
  • whole blood

Cite this

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title = "Cathodic quantum dot facilitated electrochemiluminescent detection",
abstract = "The expansion of electrochemical sensors to biomedical applications at point of care requires these sensors to undergo analysis without any pre-treatment of extraction. This poses a major challenge for all electrochemical sensors including electrochemiluminescent (ECL) based sensors. ECL offers many advantages for biomedical applications however; obtaining results from complex matrices has proven to be a large hurdle for the application of ECL sensors within this field. This work demonstrates the potential of cathodic ECL to detect and quantify homocysteine with 0.1 nM limit of detection, which is associated with hyperhomocysteinemia, in blood. This near infrared quantum dot (NIR QD) based ECL sensor displays good linearity allowing for rapid detection and providing a basis for exploitation of ECL based sensors for biomedical diagnostics utilising homocysteine as a model cathodic co-reactant. This work will lay the foundations for future developments in biosensing and imaging fields and stands as an initial proof of concept for the utilization of cathodic ECL technologies for biomedical applications once the limits of detection within clinically relevant levels has been achieved. This work illustrates the potential of cathodic ECL sensors, using Hcy as a model complex, for the detection of biomolecules.",
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Cathodic quantum dot facilitated electrochemiluminescent detection. / Stewart, Alasdair J.; Brown, Kelly; Dennany, Lynn.

In: Analytical Chemistry, Vol. 90, No. 21, 06.11.2018, p. 12944-12950.

Research output: Contribution to journalArticle

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AU - Stewart, Alasdair J.

AU - Brown, Kelly

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