Cardosin A as a model aspartic proteinase for the study of organic solvent effects. An overview on catalytic and structural aspects

A.C. Sarmento, C. Oliveira, E. Pires, P.J. Halling, M.T. Barros

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Water has proven to be one of the several limitations for broadening the scope of applications of enzymes in biocatalysis, especially when the reactants involved are poorly water-soluble. The introduction of an organic solvent into the reaction system has numerous advantages. These include a direct action of the solvents on the reactants (improving their solubility) and on the reaction products (improving or diminishing their solubility), thereby increasing the productivity of the reaction system. Nevertheless, it has been documented that the introduction of an organic solvent into the reaction mixture may induce alterations on enzyme activity, enzyme stability and thermostability, and enzyme specificity thereby imprinting new properties to old enzymes.
LanguageEnglish
Pages19-23
Number of pages4
JournalJournal of Molecular Catalysis B: Enzymatic
Volume21
Issue number1-2
DOIs
Publication statusPublished - 2003

Fingerprint

Aspartic Acid Proteases
Organic solvents
Enzymes
Solubility
Biocatalysis
Enzyme Stability
Water
Enzyme activity
Reaction products
Productivity
Peptide Hydrolases

Keywords

  • Cardosin A
  • enzyme
  • aspartic proteinase
  • organic solvent

Cite this

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Cardosin A as a model aspartic proteinase for the study of organic solvent effects. An overview on catalytic and structural aspects. / Sarmento, A.C.; Oliveira, C.; Pires, E.; Halling, P.J.; Barros, M.T.

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 21, No. 1-2, 2003, p. 19-23.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cardosin A as a model aspartic proteinase for the study of organic solvent effects. An overview on catalytic and structural aspects

AU - Sarmento, A.C.

AU - Oliveira, C.

AU - Pires, E.

AU - Halling, P.J.

AU - Barros, M.T.

PY - 2003

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KW - Cardosin A

KW - enzyme

KW - aspartic proteinase

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