Cancellous bone allograft seeded with human mesenchymal stromal cells: a potential good manufacturing practice-grade tool for the regeneration of bone defects

Maik Stiehler; F Philipp Seib; Juliane Rauh; Anja Goedecke; Carsten Werner; Martin Bornhäuser; Klaus-Peter Günther; Peter Bernstein

doi:10.3109/14653241003774052

Cancellous bone allograft seeded with human mesenchymal stromal cells: a potential good manufacturing practice-grade tool for the regeneration of bone defects

Maik Stiehler, F Philipp Seib, Juliane Rauh, Anja Goedecke, Carsten Werner, Martin Bornhäuser, Klaus-Peter Günther, Peter Bernstein

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Combining autologous bone precursor cells with cancellous bone allograft (CBA) offers an appealing strategy for skeletal regeneration. In this context, multipotent mesenchymal stromal cells (MSC) provide an excellent cell source because they are readily harvested from donors, expanded and differentiated in vitro. The aim of this study was to evaluate the proliferation, morphology, osteogenic differentiation and stem cell-related gene expression during static long-term ex vivo cultivation using human MSC and CBA under good manufacturing practice (GMP)-conforming conditions.

Original language	English
Pages (from-to)	658-668
Number of pages	11
Journal	Cytotherapy
Volume	12
Issue number	5
DOIs	https://doi.org/10.3109/14653241003774052
Publication status	Published - Sept 2010

Keywords

allograft
bone tissue engineering
cancellous bone
good manufacturing practice
human
mesenchymal stromal cell

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/14653241003774052

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title = "Cancellous bone allograft seeded with human mesenchymal stromal cells: a potential good manufacturing practice-grade tool for the regeneration of bone defects",

abstract = "Combining autologous bone precursor cells with cancellous bone allograft (CBA) offers an appealing strategy for skeletal regeneration. In this context, multipotent mesenchymal stromal cells (MSC) provide an excellent cell source because they are readily harvested from donors, expanded and differentiated in vitro. The aim of this study was to evaluate the proliferation, morphology, osteogenic differentiation and stem cell-related gene expression during static long-term ex vivo cultivation using human MSC and CBA under good manufacturing practice (GMP)-conforming conditions.",

keywords = "allograft, bone tissue engineering, cancellous bone, good manufacturing practice, human, mesenchymal stromal cell",

author = "Maik Stiehler and Seib, \{F Philipp\} and Juliane Rauh and Anja Goedecke and Carsten Werner and Martin Bornh{\"a}user and Klaus-Peter G{\"u}nther and Peter Bernstein",

year = "2010",

month = sep,

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pages = "658--668",

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TY - JOUR

T1 - Cancellous bone allograft seeded with human mesenchymal stromal cells

T2 - a potential good manufacturing practice-grade tool for the regeneration of bone defects

AU - Stiehler, Maik

AU - Seib, F Philipp

AU - Rauh, Juliane

AU - Goedecke, Anja

AU - Werner, Carsten

AU - Bornhäuser, Martin

AU - Günther, Klaus-Peter

AU - Bernstein, Peter

PY - 2010/9

Y1 - 2010/9

N2 - Combining autologous bone precursor cells with cancellous bone allograft (CBA) offers an appealing strategy for skeletal regeneration. In this context, multipotent mesenchymal stromal cells (MSC) provide an excellent cell source because they are readily harvested from donors, expanded and differentiated in vitro. The aim of this study was to evaluate the proliferation, morphology, osteogenic differentiation and stem cell-related gene expression during static long-term ex vivo cultivation using human MSC and CBA under good manufacturing practice (GMP)-conforming conditions.

AB - Combining autologous bone precursor cells with cancellous bone allograft (CBA) offers an appealing strategy for skeletal regeneration. In this context, multipotent mesenchymal stromal cells (MSC) provide an excellent cell source because they are readily harvested from donors, expanded and differentiated in vitro. The aim of this study was to evaluate the proliferation, morphology, osteogenic differentiation and stem cell-related gene expression during static long-term ex vivo cultivation using human MSC and CBA under good manufacturing practice (GMP)-conforming conditions.

KW - allograft

KW - bone tissue engineering

KW - cancellous bone

KW - good manufacturing practice

KW - human

KW - mesenchymal stromal cell

U2 - 10.3109/14653241003774052

DO - 10.3109/14653241003774052

M3 - Article

C2 - 20429788

VL - 12

SP - 658

EP - 668

JO - Cytotherapy

JF - Cytotherapy

IS - 5

ER -

Cancellous bone allograft seeded with human mesenchymal stromal cells: a potential good manufacturing practice-grade tool for the regeneration of bone defects

Abstract

Keywords

UN SDGs

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