Cancellous bone allograft seeded with human mesenchymal stromal cells: a potential good manufacturing practice-grade tool for the regeneration of bone defects

Maik Stiehler, F Philipp Seib, Juliane Rauh, Anja Goedecke, Carsten Werner, Martin Bornhäuser, Klaus-Peter Günther, Peter Bernstein

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Combining autologous bone precursor cells with cancellous bone allograft (CBA) offers an appealing strategy for skeletal regeneration. In this context, multipotent mesenchymal stromal cells (MSC) provide an excellent cell source because they are readily harvested from donors, expanded and differentiated in vitro. The aim of this study was to evaluate the proliferation, morphology, osteogenic differentiation and stem cell-related gene expression during static long-term ex vivo cultivation using human MSC and CBA under good manufacturing practice (GMP)-conforming conditions.
Original languageEnglish
Pages (from-to)658-668
Number of pages11
JournalCytotherapy
Volume12
Issue number5
DOIs
Publication statusPublished - Sep 2010

Keywords

  • allograft
  • bone tissue engineering
  • cancellous bone
  • good manufacturing practice
  • human
  • mesenchymal stromal cell

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