Cancellous bone allograft seeded with human mesenchymal stromal cells

a potential good manufacturing practice-grade tool for the regeneration of bone defects

Maik Stiehler, F Philipp Seib, Juliane Rauh, Anja Goedecke, Carsten Werner, Martin Bornhäuser, Klaus-Peter Günther, Peter Bernstein

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Combining autologous bone precursor cells with cancellous bone allograft (CBA) offers an appealing strategy for skeletal regeneration. In this context, multipotent mesenchymal stromal cells (MSC) provide an excellent cell source because they are readily harvested from donors, expanded and differentiated in vitro. The aim of this study was to evaluate the proliferation, morphology, osteogenic differentiation and stem cell-related gene expression during static long-term ex vivo cultivation using human MSC and CBA under good manufacturing practice (GMP)-conforming conditions.
Original languageEnglish
Pages (from-to)658-668
Number of pages11
JournalCytotherapy
Volume12
Issue number5
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Bone Regeneration
Mesenchymal Stromal Cells
Allografts
Regeneration
Stem Cells
Gene Expression
Bone and Bones
Cancellous Bone
In Vitro Techniques

Keywords

  • allograft
  • bone tissue engineering
  • cancellous bone
  • good manufacturing practice
  • human
  • mesenchymal stromal cell

Cite this

Stiehler, Maik ; Seib, F Philipp ; Rauh, Juliane ; Goedecke, Anja ; Werner, Carsten ; Bornhäuser, Martin ; Günther, Klaus-Peter ; Bernstein, Peter. / Cancellous bone allograft seeded with human mesenchymal stromal cells : a potential good manufacturing practice-grade tool for the regeneration of bone defects. In: Cytotherapy. 2010 ; Vol. 12, No. 5. pp. 658-668.
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Cancellous bone allograft seeded with human mesenchymal stromal cells : a potential good manufacturing practice-grade tool for the regeneration of bone defects. / Stiehler, Maik; Seib, F Philipp; Rauh, Juliane; Goedecke, Anja; Werner, Carsten; Bornhäuser, Martin; Günther, Klaus-Peter; Bernstein, Peter.

In: Cytotherapy, Vol. 12, No. 5, 09.2010, p. 658-668.

Research output: Contribution to journalArticle

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AU - Stiehler, Maik

AU - Seib, F Philipp

AU - Rauh, Juliane

AU - Goedecke, Anja

AU - Werner, Carsten

AU - Bornhäuser, Martin

AU - Günther, Klaus-Peter

AU - Bernstein, Peter

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KW - allograft

KW - bone tissue engineering

KW - cancellous bone

KW - good manufacturing practice

KW - human

KW - mesenchymal stromal cell

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DO - 10.3109/14653241003774052

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