Ca(2)+-stimulated exocytosis in maize coleoptile cells

J U Sutter, U Homann, G Thiel

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Changes in membrane capacitance (C(m)) after photolysis of the caged Ca(2)+ compound dimethoxynitrophenamine were studied in protoplasts from maize coleoptiles. Changes in C(m) values resulting from increased concentrations of free Ca(2)+ in the cytoplasm ([Ca(2)+](cyt)) were interpreted as representing changes in [Ca(2)+](cyt)-sensitive exocytosis and endocytosis. A continuous increase in [Ca(2)+](cyt) resulted in a sigmoidal increase in C(m) values with a half-maximal concentration at approximately 1 microM. The steep increase in C(m) values was followed by a variable slow phase in changing C(m) values. When [Ca(2)+](cyt) increased at a rate of 0.6 micromol L(-)(1) sec(-)(1), the initial steep increase in C(m) values lasted approximately 5 to 10 sec. During this time, protoplasts increased in surface area by approximately 2.5%. The biphasic dynamics of [Ca(2)+](cyt)-stimulated increases in C(m) values can be described by a kinetic model containing two pools of vesicles with two [Ca(2)+](cyt)-sensitive steps in the exocytotic pathway.
LanguageEnglish
Pages1127-1136
Number of pages10
JournalPlant Cell
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 2000

Fingerprint

exocytosis
Cotyledon
Protoplasts
Exocytosis
coleoptiles
protoplasts
Zea mays
capacitance
corn
Photolysis
photolysis
endocytosis
Endocytosis
surface area
Cytoplasm
cytoplasm
cells
kinetics
Membranes

Keywords

  • membrane capacitance
  • photolysis
  • dimethoxynitrophenamine

Cite this

Sutter, J U ; Homann, U ; Thiel, G. / Ca(2)+-stimulated exocytosis in maize coleoptile cells. In: Plant Cell. 2000 ; Vol. 12, No. 7. pp. 1127-1136.
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Ca(2)+-stimulated exocytosis in maize coleoptile cells. / Sutter, J U; Homann, U; Thiel, G.

In: Plant Cell, Vol. 12, No. 7, 07.2000, p. 1127-1136.

Research output: Contribution to journalArticle

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T1 - Ca(2)+-stimulated exocytosis in maize coleoptile cells

AU - Sutter, J U

AU - Homann, U

AU - Thiel, G

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AB - Changes in membrane capacitance (C(m)) after photolysis of the caged Ca(2)+ compound dimethoxynitrophenamine were studied in protoplasts from maize coleoptiles. Changes in C(m) values resulting from increased concentrations of free Ca(2)+ in the cytoplasm ([Ca(2)+](cyt)) were interpreted as representing changes in [Ca(2)+](cyt)-sensitive exocytosis and endocytosis. A continuous increase in [Ca(2)+](cyt) resulted in a sigmoidal increase in C(m) values with a half-maximal concentration at approximately 1 microM. The steep increase in C(m) values was followed by a variable slow phase in changing C(m) values. When [Ca(2)+](cyt) increased at a rate of 0.6 micromol L(-)(1) sec(-)(1), the initial steep increase in C(m) values lasted approximately 5 to 10 sec. During this time, protoplasts increased in surface area by approximately 2.5%. The biphasic dynamics of [Ca(2)+](cyt)-stimulated increases in C(m) values can be described by a kinetic model containing two pools of vesicles with two [Ca(2)+](cyt)-sensitive steps in the exocytotic pathway.

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