Ca2+ microdomains in smooth muscle

John G. McCarron, Susan Chalmers, Karen N. Bradley, Debbi MacMillan, Thomas C. Muir

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

In smooth muscle, Ca2+ controls diverse activities including cell division, contraction and cell death. Of particular significance in enabling Ca2+ to perform these multiple functions is the cell's ability to localize Ca2+ signals to certain regions by creating high local concentrations of Ca2+ (microdomains), which differ from the cytoplasmic average. Microdomains arise from Ca2+ influx across the plasma membrane or release from the sarcoplasmic reticulum (SR) Ca2+ store. A single Ca2+ channel can create a microdomain of several micromolar near (not, vert, similar200 nm) the channel. This concentration declines quickly with peak rates of several thousand micromolar per second when influx ends. The high [Ca2+] and the rapid rates of decline target Ca2+ signals to effectors in the microdomain with rapid kinetics and enable the selective activation of cellular processes. Several elements within the cell combine to enable microdomains to develop. These include the brief open time of ion channels, localization of Ca2+ by buffering, the clustering of ion channels to certain regions of the cell and the presence of membrane barriers, which restrict the free diffusion of Ca2+. In this review, the generation of microdomains arising from Ca2+ influx across the plasma membrane and the release of the ion from the SR Ca2+ store will be discussed and the contribution of mitochondria and the Golgi apparatus as well as endogenous modulators (e.g. cADPR and channel binding proteins) will be considered.
LanguageEnglish
Pages461-493
Number of pages32
JournalCell Calcium
Volume40
Issue number5-6
DOIs
Publication statusPublished - 2006

Fingerprint

Smooth Muscle
Cell Membrane
Sarcoplasmic Reticulum
Ion Channels
Cyclic ADP-Ribose
Golgi Apparatus
Cell Division
Cluster Analysis
Carrier Proteins
Mitochondria
Cell Death
Ions

Keywords

  • Ca2+ microdomains
  • membrane barriers
  • smooth muscle
  • Ca2+ signalling

Cite this

McCarron, John G. ; Chalmers, Susan ; Bradley, Karen N. ; MacMillan, Debbi ; Muir, Thomas C. / Ca2+ microdomains in smooth muscle. In: Cell Calcium. 2006 ; Vol. 40, No. 5-6. pp. 461-493.
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McCarron, JG, Chalmers, S, Bradley, KN, MacMillan, D & Muir, TC 2006, 'Ca2+ microdomains in smooth muscle' Cell Calcium, vol. 40, no. 5-6, pp. 461-493. https://doi.org/10.1016/j.ceca.2006.08.010

Ca2+ microdomains in smooth muscle. / McCarron, John G.; Chalmers, Susan; Bradley, Karen N.; MacMillan, Debbi; Muir, Thomas C.

In: Cell Calcium, Vol. 40, No. 5-6, 2006, p. 461-493.

Research output: Contribution to journalArticle

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AU - McCarron, John G.

AU - Chalmers, Susan

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AU - MacMillan, Debbi

AU - Muir, Thomas C.

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