Branched peptide actuators for enzyme responsive hydrogel particles

Tom O. McDonald, Honglei Qu, Brian R. Saunders, Rein V. Ulijn

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We demonstrate the preparation of enzyme responsive poly(ethylene glycol) acrylamide hydrogel microparticles (mu PEGA) functionalised by solid phase synthesis with new branched peptide actuators. Branched peptide actuators provide enhanced charge density and overcome electrostatic screening at physiological ionic strength when compared to linear ones which do not show triggered swelling under these conditions. Particle swelling was induced by enzymatic hydrolysis which caused a change in the charge balance of the branched peptide actuators from zwitterionic (neutral) to cationic. Analysis of enzymatic activity and accessibility was undertaken using fluorescence labelling and two-photon microscopy. These experiments revealed that thermolysin could access the core of particles when linear peptides are used, while access was restricted to the surface when using branched actuators. These responsive mPEGA particles were then loaded with a fluorescent labeled dextran by application of a sequential pH change. The payload could be selectively released at physiological ionic strength when exposed to the target enzyme.

Original languageEnglish
Pages (from-to)1728-1734
Number of pages7
JournalSoft Matter
Volume5
Issue number8
Early online date5 Mar 2009
DOIs
Publication statusPublished - 2009

Keywords

  • solid phase synthesis
  • peptide
  • hydrogels

Fingerprint Dive into the research topics of 'Branched peptide actuators for enzyme responsive hydrogel particles'. Together they form a unique fingerprint.

  • Cite this