Bovine coronavirus in neonatal calf diarrhoea in Iran

Samad Lotfollahzadeh, Omid Madadgar, Mohammad Reza Mohebbi, Mohammad Reza Mokhber Dezfouli, David George Watson

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Abstract

Partial gene sequencing for the bovine coronavirus at the World Genebank is available for many countries, which are distributed unevenly in five continents, but so far, no sequencing of strains has been recorded in Iran. One hundred ninety-four stool samples from calves with diarrhoea less than one-month old were collected from five different geographical regions of country in order to detect coronavirus and characterize it if coronavirus was found. Samples were screened for the presence of BCoV by using a commercially available ELISA kit. Furthermore, RT-PCR was carried out on positive samples for confirmation of the presence of N and S specific genes. Sequencing and phylogenetic analysis was carried out following RT-PCR tests. 7.2% of samples, were positive for BCoV and all stool samples from the South-West, Northeast and West regions of Iran were negative. The results showed that all the strains of coronavirus identified in Iran were completely in independent clusters and that they did not stand in the same cluster as any of the strains identified in other parts of the world. The strains from Iran were quite different from strains in other parts of the world but from the point of similarity these viruses showed some similarities to the European strains, such as those found in France, Croatia, Denmark and Sweden.

Original languageEnglish
Number of pages9
JournalVeterinary Medicine and Science
Early online date29 Apr 2020
DOIs
Publication statusE-pub ahead of print - 29 Apr 2020

Keywords

  • bovine coronavirus
  • calf diarrhoea
  • Iran
  • phylogenetic analysis
  • RT-PCR

Cite this

Lotfollahzadeh, S., Madadgar, O., Reza Mohebbi, M., Reza Mokhber Dezfouli, M., & Watson, D. G. (2020). Bovine coronavirus in neonatal calf diarrhoea in Iran. Veterinary Medicine and Science. https://doi.org/10.1002/vms3.277