Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D

Alan Faulkner-Jones, Catherine Fyfe, Dirk-Jan Cornelissen, John Gardner, Jason King, Aidan Courtney, Wenmiao Shu

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

We report the first investigation into the bioprinting of human induced pluripotent stem cells (hiPSCs), their response to a valve-based printing process as well as their post-printing differentiation into hepatocyte-like cells (HLCs). HLCs differentiated from both hiPSCs and human embryonic stem cells (hESCs) sources were bioprinted and examined for the presence of hepatic markers to further validate the compatibility of the valve-based bioprinting process with fragile cell transfer. Examined cells were positive for nuclear factor 4 alpha and were demonstrated to secrete albumin and have morphology that was also found to be similar to that of hepatocytes. Both hESC and hiPSC lines were tested for post-printing viability and pluripotency and were found to have negligible difference in terms of viability and pluripotency between the printed and non-printed cells. hESC-derived HLCs were 3D printed using alginate hydrogel matrix and tested for viability and albumin secretion during the remaining differentiation and were found to be hepatic in nature. 3D printed with 40-layer of HLC-containing alginate structures reached peak albumin secretion at day 21 of the differentiation protocol. This work demonstrates that the valve-based printing process is gentle enough to print human pluripotent stem cells (hPSCs) (both hESCs and hiPSCs) while either maintaining their pluripotency or directing their differentiation into specific lineages. The ability to bioprint hPSCs will pave the way for producing organs or tissues on demand from patient specific cells which could be used for animal-free drug development and personalized medicine.
LanguageEnglish
Number of pages13
JournalBiofabrication
Volume7
Issue number4
DOIs
Publication statusPublished - 21 Oct 2015

Fingerprint

Bioprinting
Pluripotent Stem Cells
Stem cells
Liver
Hepatocytes
Induced Pluripotent Stem Cells
Printing
Albumins
Alginate
Precision Medicine
Hydrogel
Hydrogels
Medicine

Keywords

  • 3D bioprinting
  • human induced pluripotent stem cells
  • human embryonic stem cells
  • stem cells
  • valve-based printing
  • hepatocyte-like cells
  • mini-liver

Cite this

Faulkner-Jones, Alan ; Fyfe, Catherine ; Cornelissen, Dirk-Jan ; Gardner, John ; King, Jason ; Courtney, Aidan ; Shu, Wenmiao. / Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D. In: Biofabrication. 2015 ; Vol. 7, No. 4.
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Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D. / Faulkner-Jones, Alan; Fyfe, Catherine; Cornelissen, Dirk-Jan; Gardner, John; King, Jason; Courtney, Aidan; Shu, Wenmiao.

In: Biofabrication, Vol. 7, No. 4, 21.10.2015.

Research output: Contribution to journalArticle

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T1 - Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D

AU - Faulkner-Jones, Alan

AU - Fyfe, Catherine

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AU - Shu, Wenmiao

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KW - human embryonic stem cells

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