Biopolymers for biosensors

polypeptide nanotubes for optical biosensing

H. Duran, K. H. A. Lau, A. Luebbert, U. Jonas, M. Steinhart, W. Knoll

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

In this work, N-carboxy anhydride (NCA) monomer molecules were condensed on the pore walls of an initiator-coated nanoporous alumina template, leading to polypeptide (poly(gamma-benzyl-L-glutamate), PBLG) film formation. Three different ways were followed for peptide nanotube formation: NCA polymerization in solution, in melt and polymerization from surface-nitiated vapor deposition. While the NCA monomer was polymerized within the pores, the wall thickness of the resulting polypeptide was tuned by changing the polymerization time. This polypeptide-coated alumina membrane will be used as planar optical waveguide to monitor both the changes in the refractive index and fluorescent signals of the composite membrane through specific binding of a bioanalyte. We monitored for the first time the in-situ formation of an initiator layer (3-Aminopropyltriethoxysilane, APTE) inside the pores of an alumina membrane via optical waveguide spectroscopy. Attachment of initiator molecule effectively changed the dielectric constants of the interfaces, resulting in detectable shifts of the waveguide modes. We have previously demonstrated that unmodified nanoporous alumina waveguide sensor having a 10 times higher sensitivity than surface plasmon spectroscopy (SPR). The sensitivity may be further increased if the pores are coated with PBLG polypeptides, which has many functional sites on each polypeptide chain.
Original languageEnglish
Title of host publicationPolymers for Biomedical Applications
EditorsA. Mahapatro, A. S. Kulshrestha
Place of PublicationSan Francisco
PublisherAmerican Chemical Society
Pages371-390
Number of pages20
ISBN (Print)9780841239661
DOIs
Publication statusPublished - 28 Mar 2008

Publication series

NameACS Symposium Series
PublisherAmerican Chemical Society
Volume977
ISSN (Print)0097-6156

Fingerprint

Biopolymers
Biosensors
Nanotubes
Aluminum Oxide
Anhydrides
Peptides
Polymerization
Optical waveguides
Peptide Nanotubes
Waveguides
Monomers
Spectroscopy
Membranes
Vapor deposition
Molecules
Planar waveguides
Composite membranes
Refractive index
Permittivity
Sensors

Keywords

  • biopolymers
  • biosensors
  • polypeptide nanotubes
  • optical biosensing

Cite this

Duran, H., Lau, K. H. A., Luebbert, A., Jonas, U., Steinhart, M., & Knoll, W. (2008). Biopolymers for biosensors: polypeptide nanotubes for optical biosensing. In A. Mahapatro, & A. S. Kulshrestha (Eds.), Polymers for Biomedical Applications (pp. 371-390). (ACS Symposium Series; Vol. 977). San Francisco: American Chemical Society. https://doi.org/10.1021/bk-2008-0977.ch022
Duran, H. ; Lau, K. H. A. ; Luebbert, A. ; Jonas, U. ; Steinhart, M. ; Knoll, W. / Biopolymers for biosensors : polypeptide nanotubes for optical biosensing. Polymers for Biomedical Applications. editor / A. Mahapatro ; A. S. Kulshrestha. San Francisco : American Chemical Society, 2008. pp. 371-390 (ACS Symposium Series).
@inbook{6a3a22383c864bcbaf42816e0af8e975,
title = "Biopolymers for biosensors: polypeptide nanotubes for optical biosensing",
abstract = "In this work, N-carboxy anhydride (NCA) monomer molecules were condensed on the pore walls of an initiator-coated nanoporous alumina template, leading to polypeptide (poly(gamma-benzyl-L-glutamate), PBLG) film formation. Three different ways were followed for peptide nanotube formation: NCA polymerization in solution, in melt and polymerization from surface-nitiated vapor deposition. While the NCA monomer was polymerized within the pores, the wall thickness of the resulting polypeptide was tuned by changing the polymerization time. This polypeptide-coated alumina membrane will be used as planar optical waveguide to monitor both the changes in the refractive index and fluorescent signals of the composite membrane through specific binding of a bioanalyte. We monitored for the first time the in-situ formation of an initiator layer (3-Aminopropyltriethoxysilane, APTE) inside the pores of an alumina membrane via optical waveguide spectroscopy. Attachment of initiator molecule effectively changed the dielectric constants of the interfaces, resulting in detectable shifts of the waveguide modes. We have previously demonstrated that unmodified nanoporous alumina waveguide sensor having a 10 times higher sensitivity than surface plasmon spectroscopy (SPR). The sensitivity may be further increased if the pores are coated with PBLG polypeptides, which has many functional sites on each polypeptide chain.",
keywords = "biopolymers , biosensors, polypeptide nanotubes, optical biosensing",
author = "H. Duran and Lau, {K. H. A.} and A. Luebbert and U. Jonas and M. Steinhart and W. Knoll",
year = "2008",
month = "3",
day = "28",
doi = "10.1021/bk-2008-0977.ch022",
language = "English",
isbn = "9780841239661",
series = "ACS Symposium Series",
publisher = "American Chemical Society",
pages = "371--390",
editor = "A. Mahapatro and Kulshrestha, {A. S.}",
booktitle = "Polymers for Biomedical Applications",
address = "United States",

}

Duran, H, Lau, KHA, Luebbert, A, Jonas, U, Steinhart, M & Knoll, W 2008, Biopolymers for biosensors: polypeptide nanotubes for optical biosensing. in A Mahapatro & AS Kulshrestha (eds), Polymers for Biomedical Applications. ACS Symposium Series, vol. 977, American Chemical Society, San Francisco, pp. 371-390. https://doi.org/10.1021/bk-2008-0977.ch022

Biopolymers for biosensors : polypeptide nanotubes for optical biosensing. / Duran, H.; Lau, K. H. A.; Luebbert, A.; Jonas, U.; Steinhart, M.; Knoll, W.

Polymers for Biomedical Applications. ed. / A. Mahapatro; A. S. Kulshrestha. San Francisco : American Chemical Society, 2008. p. 371-390 (ACS Symposium Series; Vol. 977).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Biopolymers for biosensors

T2 - polypeptide nanotubes for optical biosensing

AU - Duran, H.

AU - Lau, K. H. A.

AU - Luebbert, A.

AU - Jonas, U.

AU - Steinhart, M.

AU - Knoll, W.

PY - 2008/3/28

Y1 - 2008/3/28

N2 - In this work, N-carboxy anhydride (NCA) monomer molecules were condensed on the pore walls of an initiator-coated nanoporous alumina template, leading to polypeptide (poly(gamma-benzyl-L-glutamate), PBLG) film formation. Three different ways were followed for peptide nanotube formation: NCA polymerization in solution, in melt and polymerization from surface-nitiated vapor deposition. While the NCA monomer was polymerized within the pores, the wall thickness of the resulting polypeptide was tuned by changing the polymerization time. This polypeptide-coated alumina membrane will be used as planar optical waveguide to monitor both the changes in the refractive index and fluorescent signals of the composite membrane through specific binding of a bioanalyte. We monitored for the first time the in-situ formation of an initiator layer (3-Aminopropyltriethoxysilane, APTE) inside the pores of an alumina membrane via optical waveguide spectroscopy. Attachment of initiator molecule effectively changed the dielectric constants of the interfaces, resulting in detectable shifts of the waveguide modes. We have previously demonstrated that unmodified nanoporous alumina waveguide sensor having a 10 times higher sensitivity than surface plasmon spectroscopy (SPR). The sensitivity may be further increased if the pores are coated with PBLG polypeptides, which has many functional sites on each polypeptide chain.

AB - In this work, N-carboxy anhydride (NCA) monomer molecules were condensed on the pore walls of an initiator-coated nanoporous alumina template, leading to polypeptide (poly(gamma-benzyl-L-glutamate), PBLG) film formation. Three different ways were followed for peptide nanotube formation: NCA polymerization in solution, in melt and polymerization from surface-nitiated vapor deposition. While the NCA monomer was polymerized within the pores, the wall thickness of the resulting polypeptide was tuned by changing the polymerization time. This polypeptide-coated alumina membrane will be used as planar optical waveguide to monitor both the changes in the refractive index and fluorescent signals of the composite membrane through specific binding of a bioanalyte. We monitored for the first time the in-situ formation of an initiator layer (3-Aminopropyltriethoxysilane, APTE) inside the pores of an alumina membrane via optical waveguide spectroscopy. Attachment of initiator molecule effectively changed the dielectric constants of the interfaces, resulting in detectable shifts of the waveguide modes. We have previously demonstrated that unmodified nanoporous alumina waveguide sensor having a 10 times higher sensitivity than surface plasmon spectroscopy (SPR). The sensitivity may be further increased if the pores are coated with PBLG polypeptides, which has many functional sites on each polypeptide chain.

KW - biopolymers

KW - biosensors

KW - polypeptide nanotubes

KW - optical biosensing

U2 - 10.1021/bk-2008-0977.ch022

DO - 10.1021/bk-2008-0977.ch022

M3 - Chapter

SN - 9780841239661

T3 - ACS Symposium Series

SP - 371

EP - 390

BT - Polymers for Biomedical Applications

A2 - Mahapatro, A.

A2 - Kulshrestha, A. S.

PB - American Chemical Society

CY - San Francisco

ER -

Duran H, Lau KHA, Luebbert A, Jonas U, Steinhart M, Knoll W. Biopolymers for biosensors: polypeptide nanotubes for optical biosensing. In Mahapatro A, Kulshrestha AS, editors, Polymers for Biomedical Applications. San Francisco: American Chemical Society. 2008. p. 371-390. (ACS Symposium Series). https://doi.org/10.1021/bk-2008-0977.ch022