Abstract
| Language | English |
|---|---|
| Article number | 363 |
| Number of pages | 13 |
| Journal | Materials |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 24 Jan 2019 |
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Keywords
- electrospinning
- aligned fibrous scaffolds
- cell biology
- nanofibers
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Biological performance of electrospun polymer fibres. / Hall Barrientos, Ivan Joseph; MacKenzie, Graeme R.; Wilson, Clive G.; Lamprou, Dimitrios A.; Coats, Paul.
In: Materials, Vol. 12, No. 3, 363, 24.01.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - Biological performance of electrospun polymer fibres
AU - Hall Barrientos, Ivan Joseph
AU - MacKenzie, Graeme R.
AU - Wilson, Clive G.
AU - Lamprou, Dimitrios A.
AU - Coats, Paul
PY - 2019/1/24
Y1 - 2019/1/24
N2 - The evaluation of biological responses to polymeric scaffolds are important, given that the ideal scaffold should be biocompatible, biodegradable, promote cell adhesion and aid cell proliferation. The primary goal of this research was to measure the biological responses of cells against various polymeric and collagen electrospun scaffolds (polycaprolactone (PCL) and polylactic acid (PLA) polymers: PCL–drug, PCL–collagen–drug, PLA–drug and PLA–collagen–drug); cell proliferation was measured with a cell adhesion assay and cell viability using 5-bromo-2’-deoxyuridine (BrdU) and resazurin assays. The results demonstrated that there is a distinct lack of growth of cells against any irgasan (IRG) loaded scaffolds and far greater adhesion of cells against levofloxacin (LEVO) loaded scaffolds. Fourteen-day studies revealed a significant increase in cell growth after a 7-day period. The addition of collagen in the formulations did not promote greater cell adhesion. Cell viability studies revealed the levels of IRG used in scaffolds were toxic to cells, with the concentration used 475 times higher than the EC50 value for IRG. It was concluded that the negatively charged carboxylic acid group found in LEVO is attracting positively charged fibronectin, which in turn is attracting the cell to adhere to the adsorbed proteins on the surface of the scaffold. Overall, the biological studies examined in this paper are valuable as preliminary data for potential further studies into more complex aspects of cell behaviour with polymeric scaffolds.
AB - The evaluation of biological responses to polymeric scaffolds are important, given that the ideal scaffold should be biocompatible, biodegradable, promote cell adhesion and aid cell proliferation. The primary goal of this research was to measure the biological responses of cells against various polymeric and collagen electrospun scaffolds (polycaprolactone (PCL) and polylactic acid (PLA) polymers: PCL–drug, PCL–collagen–drug, PLA–drug and PLA–collagen–drug); cell proliferation was measured with a cell adhesion assay and cell viability using 5-bromo-2’-deoxyuridine (BrdU) and resazurin assays. The results demonstrated that there is a distinct lack of growth of cells against any irgasan (IRG) loaded scaffolds and far greater adhesion of cells against levofloxacin (LEVO) loaded scaffolds. Fourteen-day studies revealed a significant increase in cell growth after a 7-day period. The addition of collagen in the formulations did not promote greater cell adhesion. Cell viability studies revealed the levels of IRG used in scaffolds were toxic to cells, with the concentration used 475 times higher than the EC50 value for IRG. It was concluded that the negatively charged carboxylic acid group found in LEVO is attracting positively charged fibronectin, which in turn is attracting the cell to adhere to the adsorbed proteins on the surface of the scaffold. Overall, the biological studies examined in this paper are valuable as preliminary data for potential further studies into more complex aspects of cell behaviour with polymeric scaffolds.
KW - electrospinning
KW - aligned fibrous scaffolds
KW - cell biology
KW - nanofibers
UR - https://www.mdpi.com/journal/materials
U2 - 10.3390/ma12030363
DO - 10.3390/ma12030363
M3 - Article
VL - 12
JO - Materials
T2 - Materials
JF - Materials
SN - 1996-1944
IS - 3
M1 - 363
ER -