Biofluid infrared spectro-diagnostics: pre-analytical considerations for clinical applications

L. Lovergne, P. Bouzy, V. Untereiner, R. Garnotel, M. J. Baker, G. Thiéfin, G. D. Sockalingum

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Abstract

Several proof-of-concept studies on vibrational spectroscopy of biofluids have demonstrated that the methodology has promising potentials as a clinical diagnostic tool. However, these studies also show that there is lack of standardised protocol in sample handling and preparation prior to spectroscopic analysis. One of the most important sources of analytical errors is the pre-analytical phase. For the technique to be translated into clinics, it is clear that a very strict protocol needs to be established for such biological samples. This study focuses on some of the aspects of the pre-analytical phase in the development of high=throughput Fourier Transform Infrared (FTIR) spectroscopy of some of the most common biofluids such as serum, plasma and bile. Pre-analytical considerations that can impact either the samples (solvents, anti-coagulants, freeze-thaw cycles....) and/or spectroscopic analysis (sample preparation such as drying, deposit methods, volumes. substrates. operators dependence...) and consequently on the quality and the reproducibility of spectral data will be discussed in the report.
Original languageEnglish
Pages (from-to)521-537
Number of pages17
JournalFaraday Discussions
Volume187
Early online date14 Dec 2015
DOIs
Publication statusPublished - 1 Jun 2016

Keywords

  • FTIR spectroscopy
  • biofluids
  • pre-analytical requiements
  • sample handling
  • standardisation
  • quality test
  • reproducibility

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    Lovergne, L., Bouzy, P., Untereiner, V., Garnotel, R., Baker, M. J., Thiéfin, G., & Sockalingum, G. D. (2016). Biofluid infrared spectro-diagnostics: pre-analytical considerations for clinical applications. Faraday Discussions, 187, 521-537. https://doi.org/10.1039/c5fd00184f