Biofabricating multifunctional soft matter with enzymes and stimuli-responsive materials

Yi Liu, Jessica L. Terrell, Chen-Yu Tsao, Hsuan-Chen Wu, Vishal Javvaji, Eunkyoung Kim, Yi Cheng, Yifeng Wang, Rein V. Ulijn, Srinivasa R. Raghavan, Gary W. Rubloff, William E. Bentley, Gregory F. Payne

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Methods that allow soft matter to be fabricated with controlled structure and function would be beneficial for applications ranging from flexible electronics to regenerative medicine. Here, the assembly of a multifunctional gelatin matrix is demonstrated by triggering its self-assembly and then enzymatically assembling biological functionality. Triggered self-assembly relies on electrodeposition of the pH-responsive hydrogelator, 9-fluorenylmethoxycarbonyl-phenylalanine (Fmoc-Phe), in response to electrical inputs that generate a localized pH-gradient. Warm solutions of Fmoc-Phe and gelatin are co-deposited and, after cooling to room temperature, a physical gelatin network forms. Enzymatic assembly employs the cofactor-independent enzyme microbial transglutaminase (mTG) to perform two functions: crosslink the gelatin matrix to generate a thermally stable chemical gel and conjugate proteins to the matrix. To conjugate globular proteins to gelatin these proteins are engineered to have short lysine-rich or glutamine-rich fusion tags to provide accessible residues for mTG-catalysis. Viable bacteria can be co-deposited and entrapped within the crosslinked gelatin matrix and can proliferate upon subsequent incubation. These results demonstrate the potential for enlisting biological materials and mechanisms to biofabricate multifunctional soft matter.

LanguageEnglish
Pages3004-3012
Number of pages9
JournalAdvanced Functional Materials
Volume22
Issue number14
DOIs
Publication statusPublished - 24 Jul 2012

Fingerprint

gelatins
Gelatin
stimuli
enzymes
Enzymes
Proteins
Self assembly
Transglutaminases
phenylalanine
matrices
Flexible electronics
proteins
Phenylalanine
self assembly
assembly
Electrodeposition
Biological materials
Catalysis
glutamine
Bacteria

Keywords

  • biofabricating multifunctional soft matter
  • enzymes
  • stimuli-responsive materials
  • peptides
  • gelatin
  • microbial transglutaminase;quorum sensing

Cite this

Liu, Y., Terrell, J. L., Tsao, C-Y., Wu, H-C., Javvaji, V., Kim, E., ... Payne, G. F. (2012). Biofabricating multifunctional soft matter with enzymes and stimuli-responsive materials. Advanced Functional Materials, 22(14), 3004-3012. https://doi.org/10.1002/adfm.201200095
Liu, Yi ; Terrell, Jessica L. ; Tsao, Chen-Yu ; Wu, Hsuan-Chen ; Javvaji, Vishal ; Kim, Eunkyoung ; Cheng, Yi ; Wang, Yifeng ; Ulijn, Rein V. ; Raghavan, Srinivasa R. ; Rubloff, Gary W. ; Bentley, William E. ; Payne, Gregory F. / Biofabricating multifunctional soft matter with enzymes and stimuli-responsive materials. In: Advanced Functional Materials. 2012 ; Vol. 22, No. 14. pp. 3004-3012.
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Liu, Y, Terrell, JL, Tsao, C-Y, Wu, H-C, Javvaji, V, Kim, E, Cheng, Y, Wang, Y, Ulijn, RV, Raghavan, SR, Rubloff, GW, Bentley, WE & Payne, GF 2012, 'Biofabricating multifunctional soft matter with enzymes and stimuli-responsive materials' Advanced Functional Materials, vol. 22, no. 14, pp. 3004-3012. https://doi.org/10.1002/adfm.201200095

Biofabricating multifunctional soft matter with enzymes and stimuli-responsive materials. / Liu, Yi; Terrell, Jessica L.; Tsao, Chen-Yu; Wu, Hsuan-Chen; Javvaji, Vishal; Kim, Eunkyoung; Cheng, Yi; Wang, Yifeng; Ulijn, Rein V.; Raghavan, Srinivasa R.; Rubloff, Gary W.; Bentley, William E.; Payne, Gregory F.

In: Advanced Functional Materials, Vol. 22, No. 14, 24.07.2012, p. 3004-3012.

Research output: Contribution to journalArticle

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AU - Kim, Eunkyoung

AU - Cheng, Yi

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AU - Payne, Gregory F.

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