Biocompatible functionalization of polymersome surfaces: a new approach to surface immobilization and cell targeting using polymersomes

Stefan Egli, Martin G. Nussbaumer, Vimalkumar Balasubramanian, Mohamed Chami, Nico Bruns, Cornelia Palivan, Wolfgang Meier

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Vesicles assembled from amphiphilic block copolymers represent promising nanomaterials for applications that include drug delivery and surface functionalization. One essential requirement to guide such polymersomes to a desired site in vivo is conjugation of active, targeting ligands to the surface of preformed self-assemblies. Such conjugation chemistry must fulfill criteria of efficiency and selectivity, stability of the resulting bond, and biocompatibility. We have here developed a new system that achieves these criteria by simple conjugation of 4-formylbenzoate (4FB) functionalized polymersomes with 6-hydrazinonicotinate acetone hydrazone (HyNic) functionalized antibodies in aqueous buffer. The number of available amino groups on the surface of polymersomes composed of poly(dimethylsiloxane)-block-poly(2- methyloxazoline) diblock copolymers was investigated by reacting hydrophilic succinimidyl-activated fluorescent dye with polymersomes and evaluating the resulting emission intensity. To prove attachment of biomolecules to polymersomes, HyNic functionalized enhanced yellow fluorescent protein (eYFP) was attached to 4FB functionalized polymersomes, resulting in an average number of 5 eYFP molecules per polymersome. Two different polymersome-antibody conjugates were produced using either antibiotin IgG or trastuzumab. They showed specific targeting toward biotin-patterned surfaces and breast cancer cells. Overall, the polymersome-ligand platform appears promising for therapeutic and diagnostic use.

LanguageEnglish
Pages4476-4483
Number of pages8
JournalJournal of the American Chemical Society
Volume133
Issue number12
DOIs
Publication statusPublished - 30 Mar 2011

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Immobilization
Ligands
Nanostructures
Antibodies
Therapeutic Uses
Biotin
Fluorescent Dyes
Block copolymers
Buffers
Proteins
Immunoglobulin G
Breast Neoplasms
Biomolecules
Polydimethylsiloxane
Drug delivery
Biocompatibility
Nanostructured materials
Acetone
Pharmaceutical Preparations
Self assembly

Keywords

  • vesicles
  • amphiphilic block copolymers
  • polymersomes

Cite this

Egli, Stefan ; Nussbaumer, Martin G. ; Balasubramanian, Vimalkumar ; Chami, Mohamed ; Bruns, Nico ; Palivan, Cornelia ; Meier, Wolfgang. / Biocompatible functionalization of polymersome surfaces : a new approach to surface immobilization and cell targeting using polymersomes. In: Journal of the American Chemical Society . 2011 ; Vol. 133, No. 12. pp. 4476-4483.
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Biocompatible functionalization of polymersome surfaces : a new approach to surface immobilization and cell targeting using polymersomes. / Egli, Stefan; Nussbaumer, Martin G.; Balasubramanian, Vimalkumar; Chami, Mohamed; Bruns, Nico; Palivan, Cornelia; Meier, Wolfgang.

In: Journal of the American Chemical Society , Vol. 133, No. 12, 30.03.2011, p. 4476-4483.

Research output: Contribution to journalArticle

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