Biocompatibility of thermally hydrocarbonized porous silicon nanoparticles and their biodistribution in rats

Luis M. Bimbo, Mirkka Sarparanta, Hélder A. Santos, Anu J. Airaksinen, Ermei Mäkilä, Timo Laaksonen, Leena Peltonen, Vesa-Pekka Lehto, Jouni Hirvonen, Jarno Salonen

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Porous silicon (PSi) particles have been studied for the effects they elicit in Caco-2 and RAW 264.7 macrophage cells in terms of toxicity, oxidative stress, and inflammatory response. The most suitable particles were then functionalized with a novel (18)F label to assess their biodistribution after enteral and parenteral administration in a rat model. The results show that thermally hydrocarbonized porous silicon (THCPSi) nanoparticles did not induce any significant toxicity, oxidative stress, or inflammatory response in Caco-2 and RAW 264.7 macrophage cells. Fluorescently labeled nanoparticles were associated with the cells surface but were not extensively internalized. Biodistribution studies in rats using novel (18)F-labeled THCPSi nanoparticles demonstrated that the particles passed intact through the gastrointestinal tract after oral administration and were also not absorbed from a subcutaneous deposit. After intravenous administration, the particles were found mainly in the liver and spleen, indicating rapid removal from the circulation. Overall, these silicon-based nanosystems exhibit excellent in vivo stability, low cytotoxicity, and nonimmunogenic profiles, ideal for oral drug delivery purposes.

LanguageEnglish
Pages3023-3032
Number of pages10
JournalACS Nano
Volume4
Issue number6
Early online date28 May 2010
DOIs
Publication statusPublished - 22 Jun 2010
Externally publishedYes

Fingerprint

Porous silicon
biocompatibility
Silicon
porous silicon
Biocompatibility
Nanoparticles
rats
Rats
Oxidative stress
Macrophages
nanoparticles
macrophages
Toxicity
toxicity
Nanosystems
Oxidative Stress
cells
Cytotoxicity
Drug delivery
Liver

Keywords

  • administration, oral
  • animals
  • diffusion
  • hot temperature
  • hydrocarbons
  • male
  • nanoparticles
  • organ specificity
  • porosity
  • rats
  • rats, wistar
  • silicon
  • tissue distribution

Cite this

Bimbo, L. M., Sarparanta, M., Santos, H. A., Airaksinen, A. J., Mäkilä, E., Laaksonen, T., ... Salonen, J. (2010). Biocompatibility of thermally hydrocarbonized porous silicon nanoparticles and their biodistribution in rats. ACS Nano, 4(6), 3023-3032. https://doi.org/10.1021/nn901657w
Bimbo, Luis M. ; Sarparanta, Mirkka ; Santos, Hélder A. ; Airaksinen, Anu J. ; Mäkilä, Ermei ; Laaksonen, Timo ; Peltonen, Leena ; Lehto, Vesa-Pekka ; Hirvonen, Jouni ; Salonen, Jarno. / Biocompatibility of thermally hydrocarbonized porous silicon nanoparticles and their biodistribution in rats. In: ACS Nano. 2010 ; Vol. 4, No. 6. pp. 3023-3032.
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Bimbo, LM, Sarparanta, M, Santos, HA, Airaksinen, AJ, Mäkilä, E, Laaksonen, T, Peltonen, L, Lehto, V-P, Hirvonen, J & Salonen, J 2010, 'Biocompatibility of thermally hydrocarbonized porous silicon nanoparticles and their biodistribution in rats' ACS Nano, vol. 4, no. 6, pp. 3023-3032. https://doi.org/10.1021/nn901657w

Biocompatibility of thermally hydrocarbonized porous silicon nanoparticles and their biodistribution in rats. / Bimbo, Luis M.; Sarparanta, Mirkka; Santos, Hélder A.; Airaksinen, Anu J.; Mäkilä, Ermei; Laaksonen, Timo; Peltonen, Leena; Lehto, Vesa-Pekka; Hirvonen, Jouni; Salonen, Jarno.

In: ACS Nano, Vol. 4, No. 6, 22.06.2010, p. 3023-3032.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biocompatibility of thermally hydrocarbonized porous silicon nanoparticles and their biodistribution in rats

AU - Bimbo, Luis M.

AU - Sarparanta, Mirkka

AU - Santos, Hélder A.

AU - Airaksinen, Anu J.

AU - Mäkilä, Ermei

AU - Laaksonen, Timo

AU - Peltonen, Leena

AU - Lehto, Vesa-Pekka

AU - Hirvonen, Jouni

AU - Salonen, Jarno

PY - 2010/6/22

Y1 - 2010/6/22

N2 - Porous silicon (PSi) particles have been studied for the effects they elicit in Caco-2 and RAW 264.7 macrophage cells in terms of toxicity, oxidative stress, and inflammatory response. The most suitable particles were then functionalized with a novel (18)F label to assess their biodistribution after enteral and parenteral administration in a rat model. The results show that thermally hydrocarbonized porous silicon (THCPSi) nanoparticles did not induce any significant toxicity, oxidative stress, or inflammatory response in Caco-2 and RAW 264.7 macrophage cells. Fluorescently labeled nanoparticles were associated with the cells surface but were not extensively internalized. Biodistribution studies in rats using novel (18)F-labeled THCPSi nanoparticles demonstrated that the particles passed intact through the gastrointestinal tract after oral administration and were also not absorbed from a subcutaneous deposit. After intravenous administration, the particles were found mainly in the liver and spleen, indicating rapid removal from the circulation. Overall, these silicon-based nanosystems exhibit excellent in vivo stability, low cytotoxicity, and nonimmunogenic profiles, ideal for oral drug delivery purposes.

AB - Porous silicon (PSi) particles have been studied for the effects they elicit in Caco-2 and RAW 264.7 macrophage cells in terms of toxicity, oxidative stress, and inflammatory response. The most suitable particles were then functionalized with a novel (18)F label to assess their biodistribution after enteral and parenteral administration in a rat model. The results show that thermally hydrocarbonized porous silicon (THCPSi) nanoparticles did not induce any significant toxicity, oxidative stress, or inflammatory response in Caco-2 and RAW 264.7 macrophage cells. Fluorescently labeled nanoparticles were associated with the cells surface but were not extensively internalized. Biodistribution studies in rats using novel (18)F-labeled THCPSi nanoparticles demonstrated that the particles passed intact through the gastrointestinal tract after oral administration and were also not absorbed from a subcutaneous deposit. After intravenous administration, the particles were found mainly in the liver and spleen, indicating rapid removal from the circulation. Overall, these silicon-based nanosystems exhibit excellent in vivo stability, low cytotoxicity, and nonimmunogenic profiles, ideal for oral drug delivery purposes.

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KW - diffusion

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KW - hydrocarbons

KW - male

KW - nanoparticles

KW - organ specificity

KW - porosity

KW - rats

KW - rats, wistar

KW - silicon

KW - tissue distribution

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U2 - 10.1021/nn901657w

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SP - 3023

EP - 3032

JO - ACS Nano

T2 - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 6

ER -

Bimbo LM, Sarparanta M, Santos HA, Airaksinen AJ, Mäkilä E, Laaksonen T et al. Biocompatibility of thermally hydrocarbonized porous silicon nanoparticles and their biodistribution in rats. ACS Nano. 2010 Jun 22;4(6):3023-3032. https://doi.org/10.1021/nn901657w