Biocatalytic self-assembly using reversible and irreversible enzyme immobilization

Research output: Contribution to journalLetter

15 Citations (Scopus)

Abstract

Biocatalytic control of molecular self-assembly provides an effective approach for developing smart biomaterials, allowing versatile enzyme-mediated tuning of material structure and properties as well as biomedical applications. We functionalized surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localization and surface release on the self-assembly process. We show how these coatings could be conveniently used to release enzymes for bulk gelation as well as to irreversibly immobilize enzymes for localizing the self-assembly to the surface. The results provide insights to the mode of action of biocatalytic self-assembly relevant to nanofabrication and enzyme-responsive materials.
LanguageEnglish
Pages3266-3271
Number of pages6
JournalACS Applied Materials and Interfaces
Volume9
Issue number4
Early online date12 Jan 2017
DOIs
Publication statusPublished - 12 Jan 2017

Fingerprint

Enzyme immobilization
Self assembly
Enzymes
Coatings
Polyphenols
Biocompatible Materials
Gelation
Nanotechnology
Biomaterials
Tuning

Keywords

  • supramolecular chemistry
  • polydopamine
  • polyphenol
  • protein
  • bioinspired mateerial
  • surface functionalization
  • biointerfaces
  • hydrogel

Cite this

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title = "Biocatalytic self-assembly using reversible and irreversible enzyme immobilization",
abstract = "Biocatalytic control of molecular self-assembly provides an effective approach for developing smart biomaterials, allowing versatile enzyme-mediated tuning of material structure and properties as well as biomedical applications. We functionalized surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localization and surface release on the self-assembly process. We show how these coatings could be conveniently used to release enzymes for bulk gelation as well as to irreversibly immobilize enzymes for localizing the self-assembly to the surface. The results provide insights to the mode of action of biocatalytic self-assembly relevant to nanofabrication and enzyme-responsive materials.",
keywords = "supramolecular chemistry, polydopamine, polyphenol, protein, bioinspired mateerial, surface functionalization, biointerfaces, hydrogel",
author = "Conte, {M. P.} and Lau, {K. H. A.} and Ulijn, {R. V.}",
note = "This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, copyright {\circledC} American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsami.6b13162",
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Biocatalytic self-assembly using reversible and irreversible enzyme immobilization. / Conte, M. P.; Lau, K. H. A.; Ulijn, R. V.

In: ACS Applied Materials and Interfaces, Vol. 9, No. 4, 12.01.2017, p. 3266-3271.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Biocatalytic self-assembly using reversible and irreversible enzyme immobilization

AU - Conte, M. P.

AU - Lau, K. H. A.

AU - Ulijn, R. V.

N1 - This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsami.6b13162

PY - 2017/1/12

Y1 - 2017/1/12

N2 - Biocatalytic control of molecular self-assembly provides an effective approach for developing smart biomaterials, allowing versatile enzyme-mediated tuning of material structure and properties as well as biomedical applications. We functionalized surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localization and surface release on the self-assembly process. We show how these coatings could be conveniently used to release enzymes for bulk gelation as well as to irreversibly immobilize enzymes for localizing the self-assembly to the surface. The results provide insights to the mode of action of biocatalytic self-assembly relevant to nanofabrication and enzyme-responsive materials.

AB - Biocatalytic control of molecular self-assembly provides an effective approach for developing smart biomaterials, allowing versatile enzyme-mediated tuning of material structure and properties as well as biomedical applications. We functionalized surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localization and surface release on the self-assembly process. We show how these coatings could be conveniently used to release enzymes for bulk gelation as well as to irreversibly immobilize enzymes for localizing the self-assembly to the surface. The results provide insights to the mode of action of biocatalytic self-assembly relevant to nanofabrication and enzyme-responsive materials.

KW - supramolecular chemistry

KW - polydopamine

KW - polyphenol

KW - protein

KW - bioinspired mateerial

KW - surface functionalization

KW - biointerfaces

KW - hydrogel

UR - http://pubs.acs.org/journal/aamick

U2 - 10.1021/acsami.6b13162

DO - 10.1021/acsami.6b13162

M3 - Letter

VL - 9

SP - 3266

EP - 3271

JO - ACS Applied Materials and Interfaces

T2 - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 4

ER -