Biocatalytic self-assembly on magnetic nanoparticles

Maria P. Conte, Jugal Kishore Sahoo, Yousef M. Abul-Haija, K. H. Aaron Lau, Rein V. Ulijn

Research output: Contribution to journalArticle

17 Citations (Scopus)
31 Downloads (Pure)

Abstract

Combining (bio-)catalysis and molecular self-assembly provides an effective approach for the production and processing of self-assembled materials, by exploiting catalysis to direct the assembly kinetics and hence control the formation of ordered nanostructures. Applications of (bio-)catalytic self-assembly in biologically interfacing systems and in nanofabrication have recently been reported. Inspired by self-assembly in biological cells, efforts to confine catalysts on flat or patterned surfaces to exert spatial control over molecular gelator generation and nanostructure self-assembly have also emerged. Building on our previous work in the area, we demonstrate in this report the use of enzymes immobilized onto magnetic nanoparticles (NPs) to spatially localize the initiation of peptide self-assembly into nanofibers around NPs. The concept is generalized for both an equilibrium biocatalytic system that forms stable hydrogels and a non-equilibrium system that normally has a preset lifetime. Characterization of the hydrogels shows that self-assembly occurs at the site of enzyme immobilization on the NPs, to give rise to gels with a “hub-and-spoke” morphology where the nanofibers are linked through the enzyme-NP conjugates. This NP-controlled arrangement of self-assembled nanofibers enables remarkable enhancements in the shear strength of both hydrogel systems, as well as a dramatic extension of the hydrogel stability in the non-equilibrium system. We are also able to show that the use of magnetic NPs enables external control of both the formation of the hydrogel and its overall structure by application of an external magnetic field. We anticipate that the enhanced properties and stimuli-responsiveness of our NP-enzyme system will have applications ranging from nanomaterial fabrication to biomaterials and biosensing.
Original languageEnglish
Pages (from-to)3069-3075
Number of pages7
JournalACS Applied Materials and Interfaces
Volume10
Issue number3
Early online date28 Dec 2017
DOIs
Publication statusPublished - 24 Jan 2018

Keywords

  • biocatalysis
  • enzyme immobilization
  • magnetic nanoparticles
  • peptides
  • self-assembly

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    Data for: "Biocatalytic Self-Assembly on Magnetic Nanoparticles"

    Lau, K. H. A. (Supervisor), Conte, M. P. (Creator), Sahoo, J. (Creator) & Abul-Haija, Y. (Creator), University of Strathclyde, 5 Dec 2017

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    Activities

    • 3 Participation in conference
    • 1 Invited talk

    257th American Chemical Society National Meeting - Orlando

    K. H. Aaron Lau (Speaker)

    1 Apr 2019

    Activity: Participating in or organising an event typesParticipation in conference

    Invited Seminar in the Department of Materials Science and Engineering, UC Berkeley

    K. H. Aaron Lau (Invited speaker)

    8 Aug 2017

    Activity: Talk or presentation typesInvited talk

    254th American Chemical Society National Meeting Washington DC USA

    K. H. Aaron Lau (Speaker)

    23 Aug 2017

    Activity: Participating in or organising an event typesParticipation in conference

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