Biocatalytic self-assembly of nanostructured peptide microparticles using droplet microfluidics

Shuo Bai, Sisir Debnath, Kirsty Fiona Gibson, Barbara Schlicht, Lauren Bayne, Michele Zagnoni, Rein Ulijn

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Uniformly-sized, nanostructured peptide microparticles are generated by exploiting the ability of enzymes to serve (i) as catalysts, to control self-assembly within monodisperse, surfactant-stabilized water-in-oil microdroplets, and (ii) as destabilizers of emulsion interfaces, to enable facile transfer of the produced microparticles to water. This approach combines the advantages of biocatalytic self-assembly with the compartmentalization properties enabled by droplet microfluidics. Firstly, using microfluidic techniques, precursors of self-assembling peptide derivatives and enzymes are mixed in the microdroplets which upon catalytic conversion undergo molecular self-assembly into peptide particles, depending on the chemical nature of the precursors. Due to their amphiphilic nature, enzymes adsorb at the water-surfactant-oil interface of the droplets, inducing the transfer of peptide microparticles from the oil to the aqueous phase. Ultimately, through washing steps, enzymes can be removed from the microparticles which results in uniformely-sized particles composed of nanostructured aromatic peptide amphiphiles.
LanguageEnglish
Number of pages9
JournalSmall
Early online date5 Aug 2013
DOIs
Publication statusE-pub ahead of print - 5 Aug 2013

Fingerprint

Microfluidics
microparticles
Self assembly
Peptides
peptides
self assembly
enzymes
Enzymes
Oils
oils
Surface-Active Agents
Water
Surface active agents
surfactants
water
Amphiphiles
washing
assembling
Emulsions
Washing

Keywords

  • biocatalytic self-assembly
  • nanostructured peptide microparticles
  • droplet microfluidics

Cite this

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title = "Biocatalytic self-assembly of nanostructured peptide microparticles using droplet microfluidics",
abstract = "Uniformly-sized, nanostructured peptide microparticles are generated by exploiting the ability of enzymes to serve (i) as catalysts, to control self-assembly within monodisperse, surfactant-stabilized water-in-oil microdroplets, and (ii) as destabilizers of emulsion interfaces, to enable facile transfer of the produced microparticles to water. This approach combines the advantages of biocatalytic self-assembly with the compartmentalization properties enabled by droplet microfluidics. Firstly, using microfluidic techniques, precursors of self-assembling peptide derivatives and enzymes are mixed in the microdroplets which upon catalytic conversion undergo molecular self-assembly into peptide particles, depending on the chemical nature of the precursors. Due to their amphiphilic nature, enzymes adsorb at the water-surfactant-oil interface of the droplets, inducing the transfer of peptide microparticles from the oil to the aqueous phase. Ultimately, through washing steps, enzymes can be removed from the microparticles which results in uniformely-sized particles composed of nanostructured aromatic peptide amphiphiles.",
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Biocatalytic self-assembly of nanostructured peptide microparticles using droplet microfluidics. / Bai, Shuo; Debnath, Sisir; Gibson, Kirsty Fiona; Schlicht, Barbara; Bayne, Lauren; Zagnoni, Michele; Ulijn, Rein.

In: Small, 05.08.2013.

Research output: Contribution to journalArticle

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T1 - Biocatalytic self-assembly of nanostructured peptide microparticles using droplet microfluidics

AU - Bai, Shuo

AU - Debnath, Sisir

AU - Gibson, Kirsty Fiona

AU - Schlicht, Barbara

AU - Bayne, Lauren

AU - Zagnoni, Michele

AU - Ulijn, Rein

PY - 2013/8/5

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AB - Uniformly-sized, nanostructured peptide microparticles are generated by exploiting the ability of enzymes to serve (i) as catalysts, to control self-assembly within monodisperse, surfactant-stabilized water-in-oil microdroplets, and (ii) as destabilizers of emulsion interfaces, to enable facile transfer of the produced microparticles to water. This approach combines the advantages of biocatalytic self-assembly with the compartmentalization properties enabled by droplet microfluidics. Firstly, using microfluidic techniques, precursors of self-assembling peptide derivatives and enzymes are mixed in the microdroplets which upon catalytic conversion undergo molecular self-assembly into peptide particles, depending on the chemical nature of the precursors. Due to their amphiphilic nature, enzymes adsorb at the water-surfactant-oil interface of the droplets, inducing the transfer of peptide microparticles from the oil to the aqueous phase. Ultimately, through washing steps, enzymes can be removed from the microparticles which results in uniformely-sized particles composed of nanostructured aromatic peptide amphiphiles.

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