Biocatalytic self-assembly of 2D peptide-based nanostructures

M. Hughes, haixia Xu, Pim Frederix, A.M. Smith, Neil Hunt, Tell Tuttle, I.A. Kinloch, Rein Vincent Ulijn

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Peptide based 2D nanostructures of micronscale size in both X and Y dimensions are extremely rare because amino acid chirality favours helical structures, and nucleation-growth mechanisms usually favour uni-directional growth. We demonstrate the production of extended two-dimensional (2D) peptide nanostructures via the thermolysin catalysed condensation of Fmoc protected hydrophilic amino acid (serine, Fmoc-S) and a hydrophobic amino acid ester (phenylalanine, F-OMe). We propose that lateral self-assembly is enabled by the reversible nature of the system, favouring the thermodynamic product (extended sheets) over kinetically favoured 1 dimensional structures. Fmoc-SF-OMe forms extended arrays of β-sheet structures interlock via π-stacking between Fmoc groups. We propose that, due to its alternating hydrophilic/hydrophobic amino acid sequence, amphiphilic sheets presenting either phenyl or hydroxyl functionality are formed that assemble pair-wise, thereby shielding hydrophobic groups from the aqueous environment. Formation of these structures was supported by fluorescence emission spectroscopy, FTIR and XRD analysis and molecular mechanics minimization. At enhanced enzyme concentrations, hierarchical self-assembly was observed giving rise to spherulitic structures, with the number of spherulites dictated by enzyme concentration.
LanguageEnglish
Pages10032-10038
Number of pages7
JournalSoft Matter
Volume7
Issue number21
Early online date6 Sep 2011
DOIs
Publication statusPublished - 2011

Fingerprint

Self assembly
peptides
amino acids
self assembly
Nanostructures
Amino Acids
Peptides
enzymes
Thermolysin
spherulites
Molecular mechanics
phenylalanine
Chirality
Fluorescence spectroscopy
Emission spectroscopy
Enzymes
Phenylalanine
chirality
Hydroxyl Radical
Shielding

Keywords

  • peptide-based nanostructures
  • nanostructures
  • spherulites
  • TIC - Bionanotechnology

Cite this

Hughes, M. ; Xu, haixia ; Frederix, Pim ; Smith, A.M. ; Hunt, Neil ; Tuttle, Tell ; Kinloch, I.A. ; Ulijn, Rein Vincent. / Biocatalytic self-assembly of 2D peptide-based nanostructures. In: Soft Matter. 2011 ; Vol. 7, No. 21. pp. 10032-10038.
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Hughes, M, Xu, H, Frederix, P, Smith, AM, Hunt, N, Tuttle, T, Kinloch, IA & Ulijn, RV 2011, 'Biocatalytic self-assembly of 2D peptide-based nanostructures' Soft Matter, vol. 7, no. 21, pp. 10032-10038. https://doi.org/10.1039/C1SM05981E

Biocatalytic self-assembly of 2D peptide-based nanostructures. / Hughes, M.; Xu, haixia; Frederix, Pim; Smith, A.M.; Hunt, Neil; Tuttle, Tell; Kinloch, I.A.; Ulijn, Rein Vincent.

In: Soft Matter, Vol. 7, No. 21, 2011, p. 10032-10038.

Research output: Contribution to journalArticle

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AU - Kinloch, I.A.

AU - Ulijn, Rein Vincent

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Hughes M, Xu H, Frederix P, Smith AM, Hunt N, Tuttle T et al. Biocatalytic self-assembly of 2D peptide-based nanostructures. Soft Matter. 2011;7(21):10032-10038. https://doi.org/10.1039/C1SM05981E