Biocatalytic atom transfer radical polymerization in a protein cage nanoreactor

Kasper Renggli, Nora Sauter, Martin Rother, Martin G. Nussbaumer, Raphael Urbani, Thomas Pfohl, Nico Bruns

Research output: Contribution to journalArticle

19 Citations (Scopus)
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Abstract

Incorporation of the ATRP-catalyzing enzyme horseradish peroxidase (HRP) into the cavities of the group II chaperonin thermosome is demonstrated. The resulting nanoreactor was used to polymerize an acrylate under ARGET ATRP conditions. The confined space within the protein cage results in poly(ethylene glycol) methyl ether acrylate (PEGA) with lower molecular weights (poly(styrene)-apparent Mn = 4400 g mol-1) as well as narrower molecular weight distributions (Đ = 1.08) compared to polymerizations with the free ATRPase (Mn = 43700 g mol-1 and a Đ of 1.23).

Original languageEnglish
Pages (from-to)2133-2136
Number of pages4
JournalPolymer Chemistry
Volume8
Issue number14
Early online date13 Mar 2017
DOIs
Publication statusPublished - 14 Apr 2017

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Nanoreactors
Atom transfer radical polymerization
Polymerization
Thermosomes
Group II Chaperonins
Molecular Weight
Confined Spaces
Proteins
Methyl Ethers
Styrene
Ethylene Glycol
Horseradish Peroxidase
Molecular weight distribution
Polyethylene glycols
Ethers
Enzymes
Molecular weight
acrylic acid
methyl acrylate

Keywords

  • horseradish peroxidase
  • protein cages
  • polymerization

Cite this

Renggli, K., Sauter, N., Rother, M., Nussbaumer, M. G., Urbani, R., Pfohl, T., & Bruns, N. (2017). Biocatalytic atom transfer radical polymerization in a protein cage nanoreactor. Polymer Chemistry, 8(14), 2133-2136. https://doi.org/10.1039/c6py02155g
Renggli, Kasper ; Sauter, Nora ; Rother, Martin ; Nussbaumer, Martin G. ; Urbani, Raphael ; Pfohl, Thomas ; Bruns, Nico. / Biocatalytic atom transfer radical polymerization in a protein cage nanoreactor. In: Polymer Chemistry. 2017 ; Vol. 8, No. 14. pp. 2133-2136.
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Renggli, K, Sauter, N, Rother, M, Nussbaumer, MG, Urbani, R, Pfohl, T & Bruns, N 2017, 'Biocatalytic atom transfer radical polymerization in a protein cage nanoreactor', Polymer Chemistry, vol. 8, no. 14, pp. 2133-2136. https://doi.org/10.1039/c6py02155g

Biocatalytic atom transfer radical polymerization in a protein cage nanoreactor. / Renggli, Kasper; Sauter, Nora; Rother, Martin; Nussbaumer, Martin G.; Urbani, Raphael; Pfohl, Thomas; Bruns, Nico.

In: Polymer Chemistry, Vol. 8, No. 14, 14.04.2017, p. 2133-2136.

Research output: Contribution to journalArticle

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T1 - Biocatalytic atom transfer radical polymerization in a protein cage nanoreactor

AU - Renggli, Kasper

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AU - Rother, Martin

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AU - Pfohl, Thomas

AU - Bruns, Nico

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AB - Incorporation of the ATRP-catalyzing enzyme horseradish peroxidase (HRP) into the cavities of the group II chaperonin thermosome is demonstrated. The resulting nanoreactor was used to polymerize an acrylate under ARGET ATRP conditions. The confined space within the protein cage results in poly(ethylene glycol) methyl ether acrylate (PEGA) with lower molecular weights (poly(styrene)-apparent Mn = 4400 g mol-1) as well as narrower molecular weight distributions (Đ = 1.08) compared to polymerizations with the free ATRPase (Mn = 43700 g mol-1 and a Đ of 1.23).

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Renggli K, Sauter N, Rother M, Nussbaumer MG, Urbani R, Pfohl T et al. Biocatalytic atom transfer radical polymerization in a protein cage nanoreactor. Polymer Chemistry. 2017 Apr 14;8(14):2133-2136. https://doi.org/10.1039/c6py02155g