Biocatalysis in reaction mictures with undissolved solid substrates and products

R.V. Ulijn, I. Brazendale, G. Margetts, S.L. Flitsch, G. McConnell, J.M. Girkin, P.J. Halling

Research output: Contribution to journalArticle

Abstract

Enzymatic synthesis in reaction mixtures with mainly undissolved substrates and / or products is a synthetic strategy in which the compounds are present mostly as pure solids. It retains the main advantages of conventional enzymatic synthesis such as high regio- and stereoselectivity, absence of racemisation and reduced side-chain protection. The reaction yields are improved and the necessity to use organic solvents to shift the thermodynamic equilibrium toward synthesis is reduced by product precipitation, which makes the synthesis favourable even in water. The thermodynamics of these reaction systems have been investigated in the last few years, resulting in methods to predict the direction of a typical reaction a priori. Furthermore, studies on kinetics, enzyme concentration, pH / temperature effects, mixing and solvent selection have opened new perspectives for the understanding, modelling, optimisation and the possible large scale application of such a strategy. In this review we have tried to cover most of the literature published in the last five to ten years on biocatalysis in substrate suspensions, focusing especially on cases where the reaction products precipitate.
LanguageEnglish
Pages1339-1346
Number of pages7
JournalChemInform
Volume35
Issue number26
DOIs
Publication statusPublished - 2003

Fingerprint

Substrates
synthesis
products
Thermodynamics
Stereoselectivity
Regioselectivity
Enzyme kinetics
thermodynamic equilibrium
Reaction products
reaction products
Thermal effects
Organic solvents
Byproducts
temperature effects
enzymes
Precipitates
precipitates
Suspensions
thermodynamics
optimization

Keywords

  • stereoselectivity
  • thermodynamic
  • organic solvents
  • biocatalysis
  • biophotonics

Cite this

Ulijn, R.V. ; Brazendale, I. ; Margetts, G. ; Flitsch, S.L. ; McConnell, G. ; Girkin, J.M. ; Halling, P.J. / Biocatalysis in reaction mictures with undissolved solid substrates and products. In: ChemInform. 2003 ; Vol. 35, No. 26. pp. 1339-1346.
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Biocatalysis in reaction mictures with undissolved solid substrates and products. / Ulijn, R.V.; Brazendale, I.; Margetts, G.; Flitsch, S.L.; McConnell, G.; Girkin, J.M.; Halling, P.J.

In: ChemInform, Vol. 35, No. 26, 2003, p. 1339-1346.

Research output: Contribution to journalArticle

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