Bioactive diterpenes and sesquiterpenes from the rhizomes of Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt)

N. P. Igoli, Z. A. Obanu, A. I. Gray, C. Clements

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Abstract

Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt) is used in traditional medicines in the West and South of Africa. In the present study, the crude hexane extract of wild ginger was evaluated for in vitro bioactivity. The components isolated from the plant for the first time are: epi-curzerenone, furanodienone (sesquiterpenes), 8(17),12E-labdadiene-15,16-dial, 15-hydroxy-8(17),12E-labdadiene-16-al and 16-oxo-8(17),12E-labdadiene-15-oic acid (labdanes). Cytotoxicity determinations using five cell lines: SH-SY5Y (human, Caucasian, bone marrow, neuroblastoma), Jurkat (human, peripheral blood, leukaemia T cell), L929 (mouse, CH3/connective tissue, areolar and adipose tumour cells), Hep G2 (human, Caucasian, hepatocellular carcinoma) and Hs 27 (normal, human, foreskin cells) were carried out. Anti-trypanosomal activity against Trypanosoma brucei brucei (S427) blood stream forms and anti-bacterial activity against Mycobacterium aurum (CIP .104482) were also investigated. Activity against M. aurum was moderate and at 100μg/ml, the crude extract together with the labdanes showed specific cytotoxicity, indicating anti-cancer potency. Anti-trypanosomal activity was observed in the crude extract which increased with the pure components: 8(17),12E-labdadiene-15,16-dial (MIC = 5.3 μM) and the sesquiterpenoids (MIC = 6.9 μM) as compared to suramin activity (MIC = 10 μM). This anti-trypanosomal activity which is being reported for the first time indicates possible usage against sleeping sickness and nagana in cattle.
Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalAfrican Journal of Traditional, Complementary, and Alternative Medicines
Volume9
Issue number1
DOIs
Publication statusPublished - 2012

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Keywords

  • Siphonochilus aethiopicus
  • Labdane diterpenoids
  • cytotoxicity
  • mycobacterium-tuberculosis
  • anti-trypanosomal activity
  • alpinia
  • sesquiterpenoids
  • anti-bacterial activity
  • alamar blue assay
  • myrrh

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