Bioactive diterpenes and sesquiterpenes from the rhizomes of Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt)

N. P. Igoli, Z. A. Obanu, A. I. Gray, C. Clements

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt) is used in traditional medicines in the West and South of Africa. In the present study, the crude hexane extract of wild ginger was evaluated for in vitro bioactivity. The components isolated from the plant for the first time are: epi-curzerenone, furanodienone (sesquiterpenes), 8(17),12E-labdadiene-15,16-dial, 15-hydroxy-8(17),12E-labdadiene-16-al and 16-oxo-8(17),12E-labdadiene-15-oic acid (labdanes). Cytotoxicity determinations using five cell lines: SH-SY5Y (human, Caucasian, bone marrow, neuroblastoma), Jurkat (human, peripheral blood, leukaemia T cell), L929 (mouse, CH3/connective tissue, areolar and adipose tumour cells), Hep G2 (human, Caucasian, hepatocellular carcinoma) and Hs 27 (normal, human, foreskin cells) were carried out. Anti-trypanosomal activity against Trypanosoma brucei brucei (S427) blood stream forms and anti-bacterial activity against Mycobacterium aurum (CIP .104482) were also investigated. Activity against M. aurum was moderate and at 100μg/ml, the crude extract together with the labdanes showed specific cytotoxicity, indicating anti-cancer potency. Anti-trypanosomal activity was observed in the crude extract which increased with the pure components: 8(17),12E-labdadiene-15,16-dial (MIC = 5.3 μM) and the sesquiterpenoids (MIC = 6.9 μM) as compared to suramin activity (MIC = 10 μM). This anti-trypanosomal activity which is being reported for the first time indicates possible usage against sleeping sickness and nagana in cattle.
LanguageEnglish
Pages88-93
Number of pages6
JournalAfrican Journal of Traditional, Complementary, and Alternative Medicines
Volume9
Issue number1
DOIs
Publication statusPublished - 2012

Fingerprint

Asarum
Rhizome
Sesquiterpenes
Diterpenes
Complex Mixtures
Namibia
African Trypanosomiasis
T-Cell Leukemia
Plant Structures
Foreskin
Suramin
Trypanosoma brucei brucei
Hep G2 Cells
Hexanes
Traditional Medicine
Mycobacterium
Neuroblastoma
Connective Tissue
Hepatocellular Carcinoma
Neoplasms

Keywords

  • Siphonochilus aethiopicus
  • Labdane diterpenoids
  • cytotoxicity
  • mycobacterium-tuberculosis
  • anti-trypanosomal activity
  • alpinia
  • sesquiterpenoids
  • anti-bacterial activity
  • alamar blue assay
  • myrrh

Cite this

@article{b0deb2ef879349adb6e8300d61fac80a,
title = "Bioactive diterpenes and sesquiterpenes from the rhizomes of Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt)",
abstract = "Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt) is used in traditional medicines in the West and South of Africa. In the present study, the crude hexane extract of wild ginger was evaluated for in vitro bioactivity. The components isolated from the plant for the first time are: epi-curzerenone, furanodienone (sesquiterpenes), 8(17),12E-labdadiene-15,16-dial, 15-hydroxy-8(17),12E-labdadiene-16-al and 16-oxo-8(17),12E-labdadiene-15-oic acid (labdanes). Cytotoxicity determinations using five cell lines: SH-SY5Y (human, Caucasian, bone marrow, neuroblastoma), Jurkat (human, peripheral blood, leukaemia T cell), L929 (mouse, CH3/connective tissue, areolar and adipose tumour cells), Hep G2 (human, Caucasian, hepatocellular carcinoma) and Hs 27 (normal, human, foreskin cells) were carried out. Anti-trypanosomal activity against Trypanosoma brucei brucei (S427) blood stream forms and anti-bacterial activity against Mycobacterium aurum (CIP .104482) were also investigated. Activity against M. aurum was moderate and at 100μg/ml, the crude extract together with the labdanes showed specific cytotoxicity, indicating anti-cancer potency. Anti-trypanosomal activity was observed in the crude extract which increased with the pure components: 8(17),12E-labdadiene-15,16-dial (MIC = 5.3 μM) and the sesquiterpenoids (MIC = 6.9 μM) as compared to suramin activity (MIC = 10 μM). This anti-trypanosomal activity which is being reported for the first time indicates possible usage against sleeping sickness and nagana in cattle.",
keywords = "Siphonochilus aethiopicus, Labdane diterpenoids, cytotoxicity, mycobacterium-tuberculosis, anti-trypanosomal activity, alpinia, sesquiterpenoids, anti-bacterial activity, alamar blue assay, myrrh",
author = "Igoli, {N. P.} and Obanu, {Z. A.} and Gray, {A. I.} and C. Clements",
year = "2012",
doi = "10.4314/ajtcam.v9i1.13",
language = "English",
volume = "9",
pages = "88--93",
journal = "African Journal of Traditional, Complementary, and Alternative Medicines",
issn = "0189-6016",
publisher = "African Ethnomedicines Network",
number = "1",

}

Bioactive diterpenes and sesquiterpenes from the rhizomes of Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt). / Igoli, N. P.; Obanu, Z. A.; Gray, A. I.; Clements, C.

In: African Journal of Traditional, Complementary, and Alternative Medicines , Vol. 9, No. 1, 2012, p. 88-93.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioactive diterpenes and sesquiterpenes from the rhizomes of Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt)

AU - Igoli, N. P.

AU - Obanu, Z. A.

AU - Gray, A. I.

AU - Clements, C.

PY - 2012

Y1 - 2012

N2 - Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt) is used in traditional medicines in the West and South of Africa. In the present study, the crude hexane extract of wild ginger was evaluated for in vitro bioactivity. The components isolated from the plant for the first time are: epi-curzerenone, furanodienone (sesquiterpenes), 8(17),12E-labdadiene-15,16-dial, 15-hydroxy-8(17),12E-labdadiene-16-al and 16-oxo-8(17),12E-labdadiene-15-oic acid (labdanes). Cytotoxicity determinations using five cell lines: SH-SY5Y (human, Caucasian, bone marrow, neuroblastoma), Jurkat (human, peripheral blood, leukaemia T cell), L929 (mouse, CH3/connective tissue, areolar and adipose tumour cells), Hep G2 (human, Caucasian, hepatocellular carcinoma) and Hs 27 (normal, human, foreskin cells) were carried out. Anti-trypanosomal activity against Trypanosoma brucei brucei (S427) blood stream forms and anti-bacterial activity against Mycobacterium aurum (CIP .104482) were also investigated. Activity against M. aurum was moderate and at 100μg/ml, the crude extract together with the labdanes showed specific cytotoxicity, indicating anti-cancer potency. Anti-trypanosomal activity was observed in the crude extract which increased with the pure components: 8(17),12E-labdadiene-15,16-dial (MIC = 5.3 μM) and the sesquiterpenoids (MIC = 6.9 μM) as compared to suramin activity (MIC = 10 μM). This anti-trypanosomal activity which is being reported for the first time indicates possible usage against sleeping sickness and nagana in cattle.

AB - Wild ginger (Siphonochilus aethiopicus (Schweinf) B.L Burtt) is used in traditional medicines in the West and South of Africa. In the present study, the crude hexane extract of wild ginger was evaluated for in vitro bioactivity. The components isolated from the plant for the first time are: epi-curzerenone, furanodienone (sesquiterpenes), 8(17),12E-labdadiene-15,16-dial, 15-hydroxy-8(17),12E-labdadiene-16-al and 16-oxo-8(17),12E-labdadiene-15-oic acid (labdanes). Cytotoxicity determinations using five cell lines: SH-SY5Y (human, Caucasian, bone marrow, neuroblastoma), Jurkat (human, peripheral blood, leukaemia T cell), L929 (mouse, CH3/connective tissue, areolar and adipose tumour cells), Hep G2 (human, Caucasian, hepatocellular carcinoma) and Hs 27 (normal, human, foreskin cells) were carried out. Anti-trypanosomal activity against Trypanosoma brucei brucei (S427) blood stream forms and anti-bacterial activity against Mycobacterium aurum (CIP .104482) were also investigated. Activity against M. aurum was moderate and at 100μg/ml, the crude extract together with the labdanes showed specific cytotoxicity, indicating anti-cancer potency. Anti-trypanosomal activity was observed in the crude extract which increased with the pure components: 8(17),12E-labdadiene-15,16-dial (MIC = 5.3 μM) and the sesquiterpenoids (MIC = 6.9 μM) as compared to suramin activity (MIC = 10 μM). This anti-trypanosomal activity which is being reported for the first time indicates possible usage against sleeping sickness and nagana in cattle.

KW - Siphonochilus aethiopicus

KW - Labdane diterpenoids

KW - cytotoxicity

KW - mycobacterium-tuberculosis

KW - anti-trypanosomal activity

KW - alpinia

KW - sesquiterpenoids

KW - anti-bacterial activity

KW - alamar blue assay

KW - myrrh

U2 - 10.4314/ajtcam.v9i1.13

DO - 10.4314/ajtcam.v9i1.13

M3 - Article

VL - 9

SP - 88

EP - 93

JO - African Journal of Traditional, Complementary, and Alternative Medicines

T2 - African Journal of Traditional, Complementary, and Alternative Medicines

JF - African Journal of Traditional, Complementary, and Alternative Medicines

SN - 0189-6016

IS - 1

ER -