Projects per year
Abstract
Bile acids are known to be genotoxic and contribute to colorectal cancer (CRC). However, the link between CRC tumor bile acids to tumor location, patient sex, microbiome, immune-regulatory cells, and prognosis is not clear.
Methods
We conducted bile acid analysis using targeted liquid chromatography–mass spectrometry (LC–MS) on tumor tissues from CRC patients (n = 228) with survival analysis. We performed quantitative immunofluorescence (QIF) on tumors to examine immune cells.
Results
Twelve of the bile acids were significantly higher in right-sided colon tumors compared to left-sided colon tumors. Furthermore, in male patients, right-sided colon tumors had elevated secondary bile acids (deoxycholic acid, lithocholic acid, ursodeoxycholic acid) compared to left-sided colon tumors, but this difference between tumors by location was not observed in females. A high ratio of glycoursodeoxycholic to ursodeoxycholic was associated with 5-year overall survival (HR = 3.76, 95% CI = 1.17 to 12.1, P = 0.026), and a high ratio of glycochenodeoxycholic acid to chenodeoxycholic acid was associated with 5-year recurrence-free survival (HR = 3.61, 95% CI = 1.10 to 11.84, P = 0.034). We also show correlation between these bile acids and FoxP3 + T regulatory cells.
Conclusions
This study revealed that the distribution of bile acid abundances in colon cancer patients is tumor location-, age- and sex-specific, and are linked to patient prognosis. This study provides new implications for targeting bile acid metabolism, microbiome, and immune responses for colon cancer patients by taking into account primary tumor location and sex.
Original language | English |
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Article number | 61 |
Number of pages | 13 |
Journal | Biology of Sex Differences |
Volume | 13 |
Issue number | 1 |
DOIs | |
Publication status | Published - 23 Oct 2022 |
Keywords
- cancer
- measurement
- colon cancer
- biomarkers
- prognosis
Fingerprint
Dive into the research topics of 'Bile acid distributions, sex-specificity, and prognosis in colorectal cancer'. Together they form a unique fingerprint.Projects
- 1 Finished
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Multiscale Metrology Suite for Next-generation Healthcare Technologies (EPSRC Strategic Equipment)
Rattray, Z. (Principal Investigator), Bruns, N. (Co-investigator), Faulds, K. (Co-investigator), Graham, D. (Co-investigator), Halbert, G. (Co-investigator), Hoskins, C. (Co-investigator), McArthur, S. (Co-investigator), Perrie, Y. (Co-investigator), Reid, S. (Co-investigator) & Seib, P. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/11/21 → 31/10/24
Project: Research
Datasets
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Data for "Bile acid distributions, sex-specificity, and prognosis in colorectal cancer"
Cai, Y. (Creator), Shen, X. (Creator), Lu, L. (Creator), Yan, H. (Creator), Huang, H. (Creator), Gaule, P. (Creator), Muca, E. (Creator), Theriot, C. M. (Creator), Rattray, Z. (Creator), Rattray, N. J. W. (Creator), Lu, J. (Creator), Ahuja, N. (Creator), Zhang, Y. (Creator), Paty, P. B. (Creator), Khan, S. A. (Creator) & Johnson, C. H. (Creator), figshare, 23 May 2023
DOI: 10.6084/m9.figshare.c.6262460.v1
Dataset