TY - JOUR
T1 - Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source
AU - McConnell, G.
AU - Girkin, J.M.
AU - Poland, S.
N1 - Proceedings title: Fourth International Conference on Photonics and Imaging in Biology and Medicine.
PY - 2006/10/27
Y1 - 2006/10/27
N2 - Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. However, such lasers have several shortcomings, including the maintenance requirements, short lifetimes and high noise levels. To overcome these limitations, a light source for CLSM that is based on supercontinuum generation in photonic crystal fiber has been developed. This source provides the necessary wavelength range required to excite the widest possible variety of fluorophores. A novel method of extracting the desired wavelengths from the supercontinuum source using a digital micro-mirror device (DMD) is also described.
AB - Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. However, such lasers have several shortcomings, including the maintenance requirements, short lifetimes and high noise levels. To overcome these limitations, a light source for CLSM that is based on supercontinuum generation in photonic crystal fiber has been developed. This source provides the necessary wavelength range required to excite the widest possible variety of fluorophores. A novel method of extracting the desired wavelengths from the supercontinuum source using a digital micro-mirror device (DMD) is also described.
KW - confocal laser scanning microscopy
KW - supercontinuum generation
KW - digital micro-mirror device
KW - photonic crystal fiber
UR - http://www.10.1117/12.710991
U2 - 10.1117/12.710991
DO - 10.1117/12.710991
M3 - Article
VL - 6047
SP - 2Q1 - 2Q5
JO - Proceedings of SPIE: The International Society for Optical Engineering
JF - Proceedings of SPIE: The International Society for Optical Engineering
ER -