Projects per year
Abstract
Aggregation of the peptide beta-amyloid is known to be associated with Alzheimer's disease. According to recent findings the most neurotoxic aggregates are the oligomers formed in the initial stages of the aggregation process. Here we use beta-amyloid's (A beta's) intrinsic fluorophore tyrosine to probe the earliest peptide-to-peptide stages of aggregation, a region often merely labelled as a time lag, because negligible changes are observed by the commonly used probe ThT. Using spectrally resolved fluorescence decay time techniques and analysis we demonstrate how the distribution of 3 rotamer conformations of the single tyrosine in A beta tracks the aggregation across the time lag and beyond according to the initial peptide concentration. At low A beta concentrations
Original language | English |
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Pages (from-to) | 6434-6441 |
Number of pages | 8 |
Journal | Physical Chemistry Chemical Physics |
Volume | 13 |
Issue number | 14 |
DOIs | |
Publication status | Published - 4 Mar 2011 |
Keywords
- protein aggregation
- fibrils
- peptides
- conversion
- fibrillation
- Thioflavin-T
- alpha-synuclein
Fingerprint
Dive into the research topics of 'Beta-amyloid oligomerisation monitored by intrinsic tyrosine fluorescence'. Together they form a unique fingerprint.Projects
- 1 Finished
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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, D. (Principal Investigator), Dawson, M. (Co-investigator), Faulds, K. (Co-investigator), Girkin, J. (Co-investigator), Graham, D. (Co-investigator), Martin, R. (Co-investigator), O'Donnell, K. (Co-investigator), Rolinski, O. (Co-investigator), Smith, W. (Co-investigator) & Wynne, K. (Co-investigator)
Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)
1/08/08 → 31/01/12
Project: Research
Activities
- 1 Participation in conference
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Bit's 5th Annual Conference on Protein and Peptide
Rolinski, O. (Invited speaker)
23 Mar 2012 → 25 Mar 2012Activity: Participating in or organising an event types › Participation in conference