Bacterial S-layer preservation and rare arsenic-antimony-sulphide bioimmobilization in siliceous sediments from Champagne Pool hot spring, Waiotapu, New Zealand

Vernon R. Phoenix, Robin W. Renaut, Brian Jones, F. Grant Ferris

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Siliceous sinter, loose sediments, and suspended flocs in Champagne Pool, an anoxic hot (75 °C) spring at Waiotapu, New Zealand, are composed of opaline silica and metal-rich sulphides that contain many well-preserved, mineralized microbes. Detailed analysis by transmission electron microscopy and energy dispersive spectrometry has shown that bacterial cell wall and capsular material is preserved by the immobilization of high levels of As, Sb and S in the organic matrix. Calculation of the probable metal species in the spring water suggests that arsenic and antimony are present in solution as negative and neutrally charged sulphide or hydroxide complexes (such as HAs 2S4 -, H3As0 3 and HSb2S4 -). The early adsorption of these complexes onto reactive groups on the bacterial surface may be paramount in the excellent preservation of cell morphology. As biomineralization progresses, biomineral composition commonly becomes dominated by the precipitation of a supersaturated Al-rich amorphous silica phase. Biomineralization commonly preserves S-layers, an ordered mosaic of proteins on the outer surface of the cell wall. These are the finest ultrastructure details thus far found in microbes preserved by hydrothermal mineralization, and can be used as an aid to identify microfossils. The S-layers preserved here probably belong to Clostridium thermohydrosulfuricum or Desulfotomaculum nigrifacans.

LanguageEnglish
Pages323-331
Number of pages9
JournalJournal of the Geological Society
Volume162
Issue number2
DOIs
Publication statusPublished - 31 Mar 2005
Externally publishedYes

Fingerprint

biomineralization
antimony
thermal spring
arsenic
silica
sulfide
sinter
metal
spring water
ultrastructure
microfossil
immobilization
sediment
hydroxide
spectrometry
transmission electron microscopy
aid
mineralization
adsorption
matrix

Keywords

  • bacteria
  • biomineralization
  • hot spring
  • siliceous sinter
  • sulphides

Cite this

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title = "Bacterial S-layer preservation and rare arsenic-antimony-sulphide bioimmobilization in siliceous sediments from Champagne Pool hot spring, Waiotapu, New Zealand",
abstract = "Siliceous sinter, loose sediments, and suspended flocs in Champagne Pool, an anoxic hot (75 °C) spring at Waiotapu, New Zealand, are composed of opaline silica and metal-rich sulphides that contain many well-preserved, mineralized microbes. Detailed analysis by transmission electron microscopy and energy dispersive spectrometry has shown that bacterial cell wall and capsular material is preserved by the immobilization of high levels of As, Sb and S in the organic matrix. Calculation of the probable metal species in the spring water suggests that arsenic and antimony are present in solution as negative and neutrally charged sulphide or hydroxide complexes (such as HAs 2S4 -, H3As0 3 and HSb2S4 -). The early adsorption of these complexes onto reactive groups on the bacterial surface may be paramount in the excellent preservation of cell morphology. As biomineralization progresses, biomineral composition commonly becomes dominated by the precipitation of a supersaturated Al-rich amorphous silica phase. Biomineralization commonly preserves S-layers, an ordered mosaic of proteins on the outer surface of the cell wall. These are the finest ultrastructure details thus far found in microbes preserved by hydrothermal mineralization, and can be used as an aid to identify microfossils. The S-layers preserved here probably belong to Clostridium thermohydrosulfuricum or Desulfotomaculum nigrifacans.",
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Bacterial S-layer preservation and rare arsenic-antimony-sulphide bioimmobilization in siliceous sediments from Champagne Pool hot spring, Waiotapu, New Zealand. / Phoenix, Vernon R.; Renaut, Robin W.; Jones, Brian; Ferris, F. Grant.

In: Journal of the Geological Society, Vol. 162, No. 2, 31.03.2005, p. 323-331.

Research output: Contribution to journalArticle

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