Bacterial biomineralization: where to from here?

K. O. Konhauser, S. V. Lalonde, V. R. Phoenix

Research output: Contribution to journalReview article

15 Citations (Scopus)

Abstract

It is widely accepted that bacteria bind metals and form minerals, but where did this understanding come from? The answer is simple, Terry Beveridge. His pioneering research in the 1970s, as a PhD student at the University of Western Ontario under the supervision of Drs Robert Murray and William Fyfe, demonstrated that the outer surfaces of the common soil bacterium, Bacillus subtilis, could bind so much metal that they formed dense aggregates (e.g. Beveridge & Murray, 1976). Transition metals, in particular, imparted such strong electron-scattering power into the cell wall that some of them have subsequently been used as contrasting agents for electron microscopy (Beveridge, 1978). Indeed, many of the advances made on bacterial ultrastructure over the past three decades were made possible by metal staining and visualization of thin sections under the transmission electron microscope (TEM). From this work, another pivotal paper followed. In it, Terry used a variety of chemical treatments to modify or remove electronegative and/or electropositive groups within the cell wall of B. subtilis, and in doing so, showed that it was the anionic carboxyl groups in Gram-positive cells that contributed most to metal binding (Beveridge & Murray, 1980). By contrast, subsequent research with Gram-negative cells, such as Escherichia coli, showed that phosphate groups in the outer membrane were the main metal-binding groups (Ferris & Beveridge, 1986).
LanguageEnglish
Pages298-302
Number of pages5
JournalGeobiology
Volume6
Issue number3
Early online date22 Apr 2008
DOIs
Publication statusPublished - 30 Jun 2008
Externally publishedYes

Fingerprint

biomineralization
metal binding
metals
metal
phosphate group
electron
bacterium
Bacillus subtilis
transition element
ultrastructure
electron microscopy
thin section
transition elements
visualization
cell walls
student
scattering
transmission electron microscopes
membrane
soil bacteria

Keywords

  • metal binding
  • bacterial biomineralization

Cite this

Konhauser, K. O. ; Lalonde, S. V. ; Phoenix, V. R. / Bacterial biomineralization : where to from here?. In: Geobiology. 2008 ; Vol. 6, No. 3. pp. 298-302.
@article{0e9c955a508b4093b72b121212bd0b60,
title = "Bacterial biomineralization: where to from here?",
abstract = "It is widely accepted that bacteria bind metals and form minerals, but where did this understanding come from? The answer is simple, Terry Beveridge. His pioneering research in the 1970s, as a PhD student at the University of Western Ontario under the supervision of Drs Robert Murray and William Fyfe, demonstrated that the outer surfaces of the common soil bacterium, Bacillus subtilis, could bind so much metal that they formed dense aggregates (e.g. Beveridge & Murray, 1976). Transition metals, in particular, imparted such strong electron-scattering power into the cell wall that some of them have subsequently been used as contrasting agents for electron microscopy (Beveridge, 1978). Indeed, many of the advances made on bacterial ultrastructure over the past three decades were made possible by metal staining and visualization of thin sections under the transmission electron microscope (TEM). From this work, another pivotal paper followed. In it, Terry used a variety of chemical treatments to modify or remove electronegative and/or electropositive groups within the cell wall of B. subtilis, and in doing so, showed that it was the anionic carboxyl groups in Gram-positive cells that contributed most to metal binding (Beveridge & Murray, 1980). By contrast, subsequent research with Gram-negative cells, such as Escherichia coli, showed that phosphate groups in the outer membrane were the main metal-binding groups (Ferris & Beveridge, 1986).",
keywords = "metal binding, bacterial biomineralization",
author = "Konhauser, {K. O.} and Lalonde, {S. V.} and Phoenix, {V. R.}",
year = "2008",
month = "6",
day = "30",
doi = "10.1111/j.1472-4669.2008.00151.x",
language = "English",
volume = "6",
pages = "298--302",
journal = "Geobiology",
issn = "1472-4677",
number = "3",

}

Bacterial biomineralization : where to from here? / Konhauser, K. O.; Lalonde, S. V.; Phoenix, V. R.

In: Geobiology, Vol. 6, No. 3, 30.06.2008, p. 298-302.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Bacterial biomineralization

T2 - Geobiology

AU - Konhauser, K. O.

AU - Lalonde, S. V.

AU - Phoenix, V. R.

PY - 2008/6/30

Y1 - 2008/6/30

N2 - It is widely accepted that bacteria bind metals and form minerals, but where did this understanding come from? The answer is simple, Terry Beveridge. His pioneering research in the 1970s, as a PhD student at the University of Western Ontario under the supervision of Drs Robert Murray and William Fyfe, demonstrated that the outer surfaces of the common soil bacterium, Bacillus subtilis, could bind so much metal that they formed dense aggregates (e.g. Beveridge & Murray, 1976). Transition metals, in particular, imparted such strong electron-scattering power into the cell wall that some of them have subsequently been used as contrasting agents for electron microscopy (Beveridge, 1978). Indeed, many of the advances made on bacterial ultrastructure over the past three decades were made possible by metal staining and visualization of thin sections under the transmission electron microscope (TEM). From this work, another pivotal paper followed. In it, Terry used a variety of chemical treatments to modify or remove electronegative and/or electropositive groups within the cell wall of B. subtilis, and in doing so, showed that it was the anionic carboxyl groups in Gram-positive cells that contributed most to metal binding (Beveridge & Murray, 1980). By contrast, subsequent research with Gram-negative cells, such as Escherichia coli, showed that phosphate groups in the outer membrane were the main metal-binding groups (Ferris & Beveridge, 1986).

AB - It is widely accepted that bacteria bind metals and form minerals, but where did this understanding come from? The answer is simple, Terry Beveridge. His pioneering research in the 1970s, as a PhD student at the University of Western Ontario under the supervision of Drs Robert Murray and William Fyfe, demonstrated that the outer surfaces of the common soil bacterium, Bacillus subtilis, could bind so much metal that they formed dense aggregates (e.g. Beveridge & Murray, 1976). Transition metals, in particular, imparted such strong electron-scattering power into the cell wall that some of them have subsequently been used as contrasting agents for electron microscopy (Beveridge, 1978). Indeed, many of the advances made on bacterial ultrastructure over the past three decades were made possible by metal staining and visualization of thin sections under the transmission electron microscope (TEM). From this work, another pivotal paper followed. In it, Terry used a variety of chemical treatments to modify or remove electronegative and/or electropositive groups within the cell wall of B. subtilis, and in doing so, showed that it was the anionic carboxyl groups in Gram-positive cells that contributed most to metal binding (Beveridge & Murray, 1980). By contrast, subsequent research with Gram-negative cells, such as Escherichia coli, showed that phosphate groups in the outer membrane were the main metal-binding groups (Ferris & Beveridge, 1986).

KW - metal binding

KW - bacterial biomineralization

UR - http://www.scopus.com/inward/record.url?scp=44349193354&partnerID=8YFLogxK

UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1472-4669

U2 - 10.1111/j.1472-4669.2008.00151.x

DO - 10.1111/j.1472-4669.2008.00151.x

M3 - Review article

VL - 6

SP - 298

EP - 302

JO - Geobiology

JF - Geobiology

SN - 1472-4677

IS - 3

ER -