Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering

K. Witte, A. Rodrigo-Navarro, M. Salmeron-Sanchez

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.
Original languageEnglish
Article number100011
Number of pages9
JournalMaterials Today Bio
Volume2
Early online date18 Jun 2019
DOIs
Publication statusE-pub ahead of print - 18 Jun 2019

Keywords

  • droplet-based microfluidics
  • bioprinting
  • engineered bacteria
  • cell engineering
  • living materials
  • stem cells

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