Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies

Rui Lima, Monica Oliveira, T. Ishikawa, H. Kaji, S. Tanaka, M. Nishizawa, T. Yamaguchi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The current microdevices used for biomedical research are often manufactured using microelectromechanical systems (MEMS) technology. Although it is possible to fabricate precise and reproducible rectangular microchannels using soft lithography techniques, this kind of geometry may not reflect the actual physiology of the microcirculation. Here, we present a simple method to fabricate circular polydimethysiloxane (PDMS) microchannels aiming to mimic an in vivo microvascular environment and suitable for state-of-the-art microscale flow visualization techniques, such as confocal µPIV/PTV. By using a confocal µPTV system individual red blood cells (RBCs) were successfully tracked trough a 75 µm circular PDMS microchannel. The results show that RBC lateral dispersion increases with the volume fraction of RBCs in the solution, i.e. with the hematocrit.
LanguageEnglish
Article number035005
JournalBiofabrication
Volume1
Issue number3
DOIs
Publication statusPublished - Sep 2009

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Hemodynamics
Microchannels
Blood
Erythrocytes
Microcirculation
Physiology
Flow visualization
Hematocrit
Lithography
MEMS
Biomedical Research
Volume fraction
Cells
Technology
Geometry
In Vitro Techniques

Keywords

  • microchannels
  • polydimethysiloxane (PDMS)
  • flow visualisation

Cite this

Lima, R., Oliveira, M., Ishikawa, T., Kaji, H., Tanaka, S., Nishizawa, M., & Yamaguchi, T. (2009). Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies. Biofabrication, 1(3), [035005]. https://doi.org/10.1088/1758-5082/1/3/035005
Lima, Rui ; Oliveira, Monica ; Ishikawa, T. ; Kaji, H. ; Tanaka, S. ; Nishizawa, M. ; Yamaguchi, T. . / Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies. In: Biofabrication. 2009 ; Vol. 1, No. 3.
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Lima, R, Oliveira, M, Ishikawa, T, Kaji, H, Tanaka, S, Nishizawa, M & Yamaguchi, T 2009, 'Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies' Biofabrication, vol. 1, no. 3, 035005. https://doi.org/10.1088/1758-5082/1/3/035005

Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies. / Lima, Rui; Oliveira, Monica; Ishikawa, T.; Kaji, H.; Tanaka, S.; Nishizawa, M.; Yamaguchi, T. .

In: Biofabrication, Vol. 1, No. 3, 035005, 09.2009.

Research output: Contribution to journalArticle

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T1 - Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies

AU - Lima, Rui

AU - Oliveira, Monica

AU - Ishikawa, T.

AU - Kaji, H.

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AU - Nishizawa, M.

AU - Yamaguchi, T.

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AB - The current microdevices used for biomedical research are often manufactured using microelectromechanical systems (MEMS) technology. Although it is possible to fabricate precise and reproducible rectangular microchannels using soft lithography techniques, this kind of geometry may not reflect the actual physiology of the microcirculation. Here, we present a simple method to fabricate circular polydimethysiloxane (PDMS) microchannels aiming to mimic an in vivo microvascular environment and suitable for state-of-the-art microscale flow visualization techniques, such as confocal µPIV/PTV. By using a confocal µPTV system individual red blood cells (RBCs) were successfully tracked trough a 75 µm circular PDMS microchannel. The results show that RBC lateral dispersion increases with the volume fraction of RBCs in the solution, i.e. with the hematocrit.

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