Automated protein biomarker analysis: on-line extraction of clinical samples by molecularly imprinted polymers

Cecilia Rossetti, Magdalena Switnicka-Plak, Trine Grønhaug Halvorsen, Peter A.G. Cormack, Börje Sellergren, Léon Reubsaet

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Robust biomarker quantification is essential for the accurate diagnosis of diseases and is of great value in cancer management. In this paper, an innovative diagnostic platform is presented which provides automated molecularly imprinted solid-phase extraction (MISPE) followed by liquid chromatography-mass spectrometry (LC-MS) for biomarker determination using ProGastrin Releasing Peptide (ProGRP), a highly sensitive biomarker for Small Cell Lung Cancer, as a model. Molecularly imprinted polymer microspheres were synthesized by precipitation polymerization and analytical optimization of the most promising material led to the development of an automated quantification method for ProGRP. The method enabled analysis of patient serum samples with elevated ProGRP levels. Particularly low sample volumes were permitted using the automated extraction within a method which was time-efficient, thereby demonstrating the potential of such a strategy in a clinical setting.
LanguageEnglish
Article number44298
Number of pages11
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 17 Mar 2017

Fingerprint

Biomarkers
Polymers
Peptides
Proteins
Solid Phase Extraction
Liquid chromatography
Small Cell Lung Carcinoma
Microspheres
Liquid Chromatography
Polymerization
Mass spectrometry
Mass Spectrometry
Cells
Serum
big gastrin
Neoplasms

Keywords

  • biomarker
  • molecularly imprinted polymer
  • on-line LC_MS
  • ProGRP
  • targeted peptide analysis
  • diagnostics
  • liquid chromatography-mass spectrometry

Cite this

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abstract = "Robust biomarker quantification is essential for the accurate diagnosis of diseases and is of great value in cancer management. In this paper, an innovative diagnostic platform is presented which provides automated molecularly imprinted solid-phase extraction (MISPE) followed by liquid chromatography-mass spectrometry (LC-MS) for biomarker determination using ProGastrin Releasing Peptide (ProGRP), a highly sensitive biomarker for Small Cell Lung Cancer, as a model. Molecularly imprinted polymer microspheres were synthesized by precipitation polymerization and analytical optimization of the most promising material led to the development of an automated quantification method for ProGRP. The method enabled analysis of patient serum samples with elevated ProGRP levels. Particularly low sample volumes were permitted using the automated extraction within a method which was time-efficient, thereby demonstrating the potential of such a strategy in a clinical setting.",
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Automated protein biomarker analysis : on-line extraction of clinical samples by molecularly imprinted polymers. / Rossetti, Cecilia; Switnicka-Plak, Magdalena; Grønhaug Halvorsen, Trine; Cormack, Peter A.G.; Sellergren, Börje; Reubsaet, Léon.

In: Scientific Reports, Vol. 7, 44298, 17.03.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Automated protein biomarker analysis

T2 - Scientific Reports

AU - Rossetti, Cecilia

AU - Switnicka-Plak, Magdalena

AU - Grønhaug Halvorsen, Trine

AU - Cormack, Peter A.G.

AU - Sellergren, Börje

AU - Reubsaet, Léon

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AB - Robust biomarker quantification is essential for the accurate diagnosis of diseases and is of great value in cancer management. In this paper, an innovative diagnostic platform is presented which provides automated molecularly imprinted solid-phase extraction (MISPE) followed by liquid chromatography-mass spectrometry (LC-MS) for biomarker determination using ProGastrin Releasing Peptide (ProGRP), a highly sensitive biomarker for Small Cell Lung Cancer, as a model. Molecularly imprinted polymer microspheres were synthesized by precipitation polymerization and analytical optimization of the most promising material led to the development of an automated quantification method for ProGRP. The method enabled analysis of patient serum samples with elevated ProGRP levels. Particularly low sample volumes were permitted using the automated extraction within a method which was time-efficient, thereby demonstrating the potential of such a strategy in a clinical setting.

KW - biomarker

KW - molecularly imprinted polymer

KW - on-line LC_MS

KW - ProGRP

KW - targeted peptide analysis

KW - diagnostics

KW - liquid chromatography-mass spectrometry

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