Automated protein biomarker analysis: on-line extraction of clinical samples by molecularly imprinted polymers

Cecilia Rossetti, Magdalena Switnicka-Plak, Trine Grønhaug Halvorsen, Peter A.G. Cormack, Börje Sellergren, Léon Reubsaet

Research output: Contribution to journalArticle

13 Citations (Scopus)
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Abstract

Robust biomarker quantification is essential for the accurate diagnosis of diseases and is of great value in cancer management. In this paper, an innovative diagnostic platform is presented which provides automated molecularly imprinted solid-phase extraction (MISPE) followed by liquid chromatography-mass spectrometry (LC-MS) for biomarker determination using ProGastrin Releasing Peptide (ProGRP), a highly sensitive biomarker for Small Cell Lung Cancer, as a model. Molecularly imprinted polymer microspheres were synthesized by precipitation polymerization and analytical optimization of the most promising material led to the development of an automated quantification method for ProGRP. The method enabled analysis of patient serum samples with elevated ProGRP levels. Particularly low sample volumes were permitted using the automated extraction within a method which was time-efficient, thereby demonstrating the potential of such a strategy in a clinical setting.
Original languageEnglish
Article number44298
Number of pages11
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 17 Mar 2017

Keywords

  • biomarker
  • molecularly imprinted polymer
  • on-line LC_MS
  • ProGRP
  • targeted peptide analysis
  • diagnostics
  • liquid chromatography-mass spectrometry

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