Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin

Lucia Blanco-Covián, Verónica Montes-Garcia, Alexandre Girard, M. Teresa Fernández-Abedul, Jorge Pérez-Juste, Isabel Pastoriza-Santos, Karen Faulds, Duncan Graham, Carmen Blanco-López

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Abstract

Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core–shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL−1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.
Original languageEnglish
Pages (from-to)2051 - 2058
Number of pages8
JournalNanoscale
Volume9
Issue number5
Early online date3 Jan 2017
DOIs
Publication statusE-pub ahead of print - 3 Jan 2017

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Keywords

  • lateral flow immunoassays
  • SERRS
  • pneumolysin
  • pneumonia
  • surface enhanced resonance Raman scattering
  • point of care
  • Au@Ag

Cite this

Blanco-Covián, L., Montes-Garcia, V., Girard, A., Fernández-Abedul, M. T., Pérez-Juste, J., Pastoriza-Santos, I., ... Blanco-López, C. (2017). Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin. Nanoscale, 9(5), 2051 - 2058. https://doi.org/10.1039/C6NR08432J