Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin

Lucia Blanco-Covián, Verónica Montes-Garcia, Alexandre Girard, M. Teresa Fernández-Abedul, Jorge Pérez-Juste, Isabel Pastoriza-Santos, Karen Faulds, Duncan Graham, Carmen Blanco-López

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core–shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL−1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.
LanguageEnglish
Pages2051 - 2058
Number of pages8
JournalNanoscale
Volume9
Issue number5
Early online date3 Jan 2017
DOIs
Publication statusE-pub ahead of print - 3 Jan 2017

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Raman Spectrum Analysis
Immunoassay
Raman scattering
Pneumonia
Point-of-Care Systems
Nanoparticles
Limit of Detection
Streptococcus pneumoniae plY protein

Keywords

  • lateral flow immunoassays
  • SERRS
  • pneumolysin
  • pneumonia
  • surface enhanced resonance Raman scattering
  • point of care
  • Au@Ag

Cite this

Blanco-Covián, L., Montes-Garcia, V., Girard, A., Fernández-Abedul, M. T., Pérez-Juste, J., Pastoriza-Santos, I., ... Blanco-López, C. (2017). Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin. Nanoscale, 9(5), 2051 - 2058. https://doi.org/10.1039/C6NR08432J
Blanco-Covián, Lucia ; Montes-Garcia, Verónica ; Girard, Alexandre ; Fernández-Abedul, M. Teresa ; Pérez-Juste, Jorge ; Pastoriza-Santos, Isabel ; Faulds, Karen ; Graham, Duncan ; Blanco-López, Carmen. / Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin. In: Nanoscale. 2017 ; Vol. 9, No. 5. pp. 2051 - 2058.
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Blanco-Covián, L, Montes-Garcia, V, Girard, A, Fernández-Abedul, MT, Pérez-Juste, J, Pastoriza-Santos, I, Faulds, K, Graham, D & Blanco-López, C 2017, 'Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin' Nanoscale, vol. 9, no. 5, pp. 2051 - 2058. https://doi.org/10.1039/C6NR08432J

Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin. / Blanco-Covián, Lucia; Montes-Garcia, Verónica; Girard, Alexandre; Fernández-Abedul, M. Teresa; Pérez-Juste, Jorge; Pastoriza-Santos, Isabel; Faulds, Karen; Graham, Duncan; Blanco-López, Carmen.

In: Nanoscale, Vol. 9, No. 5, 03.01.2017, p. 2051 - 2058.

Research output: Contribution to journalArticle

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T1 - Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin

AU - Blanco-Covián, Lucia

AU - Montes-Garcia, Verónica

AU - Girard, Alexandre

AU - Fernández-Abedul, M. Teresa

AU - Pérez-Juste, Jorge

AU - Pastoriza-Santos, Isabel

AU - Faulds, Karen

AU - Graham, Duncan

AU - Blanco-López, Carmen

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Blanco-Covián L, Montes-Garcia V, Girard A, Fernández-Abedul MT, Pérez-Juste J, Pastoriza-Santos I et al. Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin. Nanoscale. 2017 Jan 3;9(5):2051 - 2058. https://doi.org/10.1039/C6NR08432J