Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin

Lucia Blanco-Covián; Verónica Montes-Garcia; Alexandre Girard; M. Teresa Fernández-Abedul; Jorge Pérez-Juste; Isabel Pastoriza-Santos; Karen Faulds; Duncan Graham; Carmen Blanco-López

doi:10.1039/C6NR08432J

Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin

Lucia Blanco-Covián, Verónica Montes-Garcia, Alexandre Girard, M. Teresa Fernández-Abedul, Jorge Pérez-Juste, Isabel Pastoriza-Santos, Karen Faulds, Duncan Graham, Carmen Blanco-López

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Abstract

Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core–shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL⁻¹. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.

Original language	English
Pages (from-to)	2051 - 2058
Number of pages	8
Journal	Nanoscale
Volume	9
Issue number	5
Early online date	3 Jan 2017
DOIs	https://doi.org/10.1039/C6NR08432J
Publication status	E-pub ahead of print - 3 Jan 2017

Keywords

lateral flow immunoassays
SERRS
pneumolysin
pneumonia
surface enhanced resonance Raman scattering
point of care
Au@Ag

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10.1039/C6NR08432J

Blanco-Covain-Nanoscale-2017-Lateral-flow-immunoassay-for-the-sensitive-detection-of-pneumolysinAccepted author manuscript, 2.31 MB

http://digibuo.uniovi.es/dspace/handle/10651/39400

Cite this

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title = "Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin",

abstract = "Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core–shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL−1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.",

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T1 - Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin

AU - Blanco-Covián, Lucia

AU - Montes-Garcia, Verónica

AU - Girard, Alexandre

AU - Fernández-Abedul, M. Teresa

AU - Pérez-Juste, Jorge

AU - Pastoriza-Santos, Isabel

AU - Faulds, Karen

AU - Graham, Duncan

AU - Blanco-López, Carmen

PY - 2017/1/3

Y1 - 2017/1/3

N2 - Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core–shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL−1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.

AB - Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core–shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL−1. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.

KW - lateral flow immunoassays

KW - SERRS

KW - pneumolysin

KW - pneumonia

KW - surface enhanced resonance Raman scattering

KW - point of care

KW - Au@Ag

U2 - 10.1039/C6NR08432J

DO - 10.1039/C6NR08432J

M3 - Article

SN - 2040-3364

VL - 9

SP - 2051

EP - 2058

JO - Nanoscale

JF - Nanoscale

IS - 5

ER -

Au@Ag SERRS tags coupled to a lateral flow immunoassay for the sensitive detection of pneumolysin

Abstract

Keywords

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