Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels.
- small RNA molecules
- surface polyadenylation reactions
Wark, A. W., Fang, S., Lee, H. J., & Corn, R. M. (2006). Attomole detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions. Journal of American Chemical Society, 126, 14044-14046. https://doi.org/10.1021/ja065223p