Attomole detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions

A.W. Wark, S. Fang, H.J. Lee, R.M. Corn

Research output: Contribution to journalArticle

367 Citations (Scopus)

Abstract

Multiple microRNAs (miRNAs) are detected in a microarray format using a novel approach that combines a surface enzyme reaction with nanoparticle-amplified SPR imaging (SPRI). The surface reaction of poly(A) polymerase creates poly(A) tails on miRNAs hybridized onto locked nucleic acid (LNA) microarrays. DNA-modified nanoparticles are then adsorbed onto the poly(A) tails and detected with SPRI. This ultrasensitive nanoparticle-amplified SPRI methodology can be used for miRNA profiling at attomole levels.
Original languageEnglish
Pages (from-to)14044-14046
Number of pages3
JournalJournal of American Chemical Society
Volume126
DOIs
Publication statusPublished - 2006

Keywords

  • MicroRNAs
  • small RNA molecules
  • DNA
  • surface polyadenylation reactions

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