Atomic force microscopy studies on two-step nucleation and epitaxial growth

Monika Warzecha, Rajni M. Bhardwaj, Susan Reutzel-Edens, Dimitrios Lamprou, Alastair Florence

Research output: Contribution to conferencePoster

Abstract

Continues advancement and rapid development of techniques operating at the nanoscale open new opportunities to revise and question commonly accepted nucleation and crystal growth theories. Atomic Force Microscopy (AFM) has been successfully involved in various aspects of active pharmaceutical ingredient (API) characterisation including crystal growth, stability of solid dispersions, surface morphology, phase changes and dissolution [1]. Recent studies conducted on proteins crystallisation at nanoscale show new evidence disproving generally accepted Classical Nuclea/on Theory (CNT)[2]. Currently, ‘dense liquid droplets’ seen in protein crystallisation and ‘pre-nucleation clusters’ [3] seen mostly in inorganic salt crystallisation, are two main concepts of non-classical nucleation theory, although no significant progress has been made towards better understanding of mechanisms controlling heterogeneous nucleation in small organic molecules systems, what is in particular interest, as an epitaxial ordering phenomenon is frequently used to enhance nucleation rates and control properties of materials. Our studies present a new light on heteronucleation and the epitaxial growth mechanisms based epitaxial growth of olanzapine dihydrate D on the surface of olanzapine form I (OZPN I) both in high humidity conditions and water solu*on. Results obtained from Peak Force Quan/ta/ve Nanomechanical Mapping Atomic Force Microscopy (PF- QNM-AFM) [4] indicate the presence of intermediate dense liquid-like phase in process of dihydrate D nucleation.

Conference

Conference46th BACG Annual Conference
CountryUnited Kingdom
CityLondon
Period21/06/1523/06/15
Internet address

Fingerprint

Atomic Force Microscopy
Crystallization
olanzapine
Growth
Humidity
Proteins
Salts
Light
Water
Pharmaceutical Preparations

Keywords

  • afm study
  • crystal growth
  • template matching
  • atomic force microscopy
  • nucleation
  • classical nucleation theory
  • dense liquid droplets
  • epitaxial growth

Cite this

Warzecha, M., Bhardwaj, R. M., Reutzel-Edens, S., Lamprou, D., & Florence, A. (2015). Atomic force microscopy studies on two-step nucleation and epitaxial growth. Poster session presented at 46th BACG Annual Conference, London, United Kingdom.
Warzecha, Monika ; Bhardwaj, Rajni M. ; Reutzel-Edens, Susan ; Lamprou, Dimitrios ; Florence, Alastair. / Atomic force microscopy studies on two-step nucleation and epitaxial growth. Poster session presented at 46th BACG Annual Conference, London, United Kingdom.1 p.
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Warzecha, M, Bhardwaj, RM, Reutzel-Edens, S, Lamprou, D & Florence, A 2015, 'Atomic force microscopy studies on two-step nucleation and epitaxial growth' 46th BACG Annual Conference, London, United Kingdom, 21/06/15 - 23/06/15, .

Atomic force microscopy studies on two-step nucleation and epitaxial growth. / Warzecha, Monika; Bhardwaj, Rajni M.; Reutzel-Edens, Susan; Lamprou, Dimitrios; Florence, Alastair.

2015. Poster session presented at 46th BACG Annual Conference, London, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Atomic force microscopy studies on two-step nucleation and epitaxial growth

AU - Warzecha, Monika

AU - Bhardwaj, Rajni M.

AU - Reutzel-Edens, Susan

AU - Lamprou, Dimitrios

AU - Florence, Alastair

PY - 2015/6/21

Y1 - 2015/6/21

N2 - Continues advancement and rapid development of techniques operating at the nanoscale open new opportunities to revise and question commonly accepted nucleation and crystal growth theories. Atomic Force Microscopy (AFM) has been successfully involved in various aspects of active pharmaceutical ingredient (API) characterisation including crystal growth, stability of solid dispersions, surface morphology, phase changes and dissolution [1]. Recent studies conducted on proteins crystallisation at nanoscale show new evidence disproving generally accepted Classical Nuclea/on Theory (CNT)[2]. Currently, ‘dense liquid droplets’ seen in protein crystallisation and ‘pre-nucleation clusters’ [3] seen mostly in inorganic salt crystallisation, are two main concepts of non-classical nucleation theory, although no significant progress has been made towards better understanding of mechanisms controlling heterogeneous nucleation in small organic molecules systems, what is in particular interest, as an epitaxial ordering phenomenon is frequently used to enhance nucleation rates and control properties of materials. Our studies present a new light on heteronucleation and the epitaxial growth mechanisms based epitaxial growth of olanzapine dihydrate D on the surface of olanzapine form I (OZPN I) both in high humidity conditions and water solu*on. Results obtained from Peak Force Quan/ta/ve Nanomechanical Mapping Atomic Force Microscopy (PF- QNM-AFM) [4] indicate the presence of intermediate dense liquid-like phase in process of dihydrate D nucleation.

AB - Continues advancement and rapid development of techniques operating at the nanoscale open new opportunities to revise and question commonly accepted nucleation and crystal growth theories. Atomic Force Microscopy (AFM) has been successfully involved in various aspects of active pharmaceutical ingredient (API) characterisation including crystal growth, stability of solid dispersions, surface morphology, phase changes and dissolution [1]. Recent studies conducted on proteins crystallisation at nanoscale show new evidence disproving generally accepted Classical Nuclea/on Theory (CNT)[2]. Currently, ‘dense liquid droplets’ seen in protein crystallisation and ‘pre-nucleation clusters’ [3] seen mostly in inorganic salt crystallisation, are two main concepts of non-classical nucleation theory, although no significant progress has been made towards better understanding of mechanisms controlling heterogeneous nucleation in small organic molecules systems, what is in particular interest, as an epitaxial ordering phenomenon is frequently used to enhance nucleation rates and control properties of materials. Our studies present a new light on heteronucleation and the epitaxial growth mechanisms based epitaxial growth of olanzapine dihydrate D on the surface of olanzapine form I (OZPN I) both in high humidity conditions and water solu*on. Results obtained from Peak Force Quan/ta/ve Nanomechanical Mapping Atomic Force Microscopy (PF- QNM-AFM) [4] indicate the presence of intermediate dense liquid-like phase in process of dihydrate D nucleation.

KW - afm study

KW - crystal growth

KW - template matching

KW - atomic force microscopy

KW - nucleation

KW - classical nucleation theory

KW - dense liquid droplets

KW - epitaxial growth

UR - http://www.bacg.co.uk/index.php/46-bacg-2015

M3 - Poster

ER -

Warzecha M, Bhardwaj RM, Reutzel-Edens S, Lamprou D, Florence A. Atomic force microscopy studies on two-step nucleation and epitaxial growth. 2015. Poster session presented at 46th BACG Annual Conference, London, United Kingdom.