Assessment of the antigen-specific antibody response induced by mucosal administration of a GnRH conjugate entrapped in lipid nanoparticles

Ayman M Gebril, Dimitrios Lamprou, Manal Alsaadi, William Stimson, Alexander Mullen, Valerie Ferro

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Abstract

Vaccines administered parenterally have been developed against gonadotrophin releasing hormone (GnRH) for anti-fertility and anti-cancer purposes. The aim of this study was to demonstrate whether mucosal delivery using GnRH immunogens entrapped in lipid nanoparticles (LNP) could induce anti-GnRH antibody titres. Immunogens consisting of KLH (keyhole limpet haemocyanin) conjugated to either GnRH-I or GnRH-III analogues were entrapped in LNP. Loaded non-ionic surfactant vesicles (NISV) were administered subcutaneously, while nasal delivery was achieved using NISV in xanthan gum and oral delivery using NISV containing deoxycholate (bilosomes). NISV and bilosomes had similar properties: they were spherical, in the nanometre size range, with a slightly negative zeta potential and surface properties that changed with protein loading and inclusion of xanthan gum. Following immunisation in female BALB/c mice, systemic antibody responses were similar for both GnRH-I and GnRH-III immunisation. Only nasal delivery proved to be successful in terms of producing systemic and mucosal antibodies.
LanguageEnglish
Pages971-979
Number of pages9
JournalNanomedicine: Nanotechnology, Biology and Medicine
Volume10
Issue number5
Early online date26 Dec 2013
DOIs
Publication statusPublished - Jul 2014

Fingerprint

Mucosal Administration
Antigens
Antibodies
Gonadotropin-Releasing Hormone
Nanoparticles
Lipids
Antibody Formation
Nonionic surfactants
Surface-Active Agents
Immunization
Xanthan gum
Nose
Deoxycholic Acid
Surface Properties
Zeta potential
Surface properties
Fertility
Vaccines
Proteins
Neoplasms

Keywords

  • gonadotrophin-releasing hormone
  • non-ionic surfactant vesicles
  • bilosomes
  • atomic force microscopy

Cite this

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abstract = "Vaccines administered parenterally have been developed against gonadotrophin releasing hormone (GnRH) for anti-fertility and anti-cancer purposes. The aim of this study was to demonstrate whether mucosal delivery using GnRH immunogens entrapped in lipid nanoparticles (LNP) could induce anti-GnRH antibody titres. Immunogens consisting of KLH (keyhole limpet haemocyanin) conjugated to either GnRH-I or GnRH-III analogues were entrapped in LNP. Loaded non-ionic surfactant vesicles (NISV) were administered subcutaneously, while nasal delivery was achieved using NISV in xanthan gum and oral delivery using NISV containing deoxycholate (bilosomes). NISV and bilosomes had similar properties: they were spherical, in the nanometre size range, with a slightly negative zeta potential and surface properties that changed with protein loading and inclusion of xanthan gum. Following immunisation in female BALB/c mice, systemic antibody responses were similar for both GnRH-I and GnRH-III immunisation. Only nasal delivery proved to be successful in terms of producing systemic and mucosal antibodies.",
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AU - Stimson, William

AU - Mullen, Alexander

AU - Ferro, Valerie

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AB - Vaccines administered parenterally have been developed against gonadotrophin releasing hormone (GnRH) for anti-fertility and anti-cancer purposes. The aim of this study was to demonstrate whether mucosal delivery using GnRH immunogens entrapped in lipid nanoparticles (LNP) could induce anti-GnRH antibody titres. Immunogens consisting of KLH (keyhole limpet haemocyanin) conjugated to either GnRH-I or GnRH-III analogues were entrapped in LNP. Loaded non-ionic surfactant vesicles (NISV) were administered subcutaneously, while nasal delivery was achieved using NISV in xanthan gum and oral delivery using NISV containing deoxycholate (bilosomes). NISV and bilosomes had similar properties: they were spherical, in the nanometre size range, with a slightly negative zeta potential and surface properties that changed with protein loading and inclusion of xanthan gum. Following immunisation in female BALB/c mice, systemic antibody responses were similar for both GnRH-I and GnRH-III immunisation. Only nasal delivery proved to be successful in terms of producing systemic and mucosal antibodies.

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