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A novel high-speed fluorescence lifetime imaging (FLIM) analysis method based on artificial neural networks (ANN) has been proposed. The proposed ANN-FLIM method does not require iterative searching procedures or initial conditions, which are usually required for traditional FLIM methods. In terms of image generation, ANN-FLIM is free from iterative computations and able to generate lifetime images at least 180-fold faster than conventional least squares curve-fitting approaches. The advantages of ANN-FLIM were demonstrated on both synthesized and experimental data, showing that it has great potential to fuel current revolutions in rapid FLIM technologies.
- fluorescence lifetime imaging microscopy