Artificial neural network approaches for fluorescence lifetime imaging techniques

Gang Wu, Thomas Nowotny, Yongliang Zhang, Hongqi Yu, David Day-Uei Li

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)
112 Downloads (Pure)


A novel high-speed fluorescence lifetime imaging (FLIM) analysis method based on artificial neural networks (ANN) has been proposed. The proposed ANN-FLIM method does not require iterative searching procedures or initial conditions, which are usually required for traditional FLIM methods. In terms of image generation, ANN-FLIM is free from iterative computations and able to generate lifetime images at least 180-fold faster than conventional least squares curve-fitting approaches. The advantages of ANN-FLIM were demonstrated on both synthesized and experimental data, showing that it has great potential to fuel current revolutions in rapid FLIM technologies.
Original languageEnglish
Pages (from-to)2561-2564
Number of pages4
JournalOptics Letters
Issue number11
Publication statusPublished - 25 May 2016


  • fluorescence lifetime imaging microscopy
  • FLIM
  • fluorophores


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