Artificial bilayer lipid membranes (BLMs) on-chip for single molecule sensing

Mairi E. Sandison, Daniele Malleo, David Holmes, Richard Berry, Hywel Morgan

Research output: Contribution to journalConference article

2 Citations (Scopus)

Abstract

A polymer microfluidic device for the formation of artificial bilayer lipid membranes (BLMs) on-chip is described. The device is fabricated from thin, transparent films of poly(methyl methacrylate), allowing for optical monitoring of the BLM. In addition, detection of single fluorescently-labeled lipid molecules using conventional epifluorescence microscopy is described.

Original languageEnglish
Article number47
Pages (from-to)252-257
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5838
DOIs
Publication statusPublished - 9 Dec 2005
EventNanotechnology II - Seville, Spain
Duration: 9 May 200511 May 2005

Fingerprint

Lipid Bilayer
Lipid bilayers
Membrane Lipids
lipids
Chip
Sensing
Membrane
chips
membranes
Molecules
Microfluidics
Polymethyl Methacrylate
Lipids
Polymethyl methacrylates
Microscopy
molecules
Microscopic examination
Polymers
microfluidic devices
Monitoring

Keywords

  • lipid bilayers
  • microfluidics
  • rapid fabrication
  • single molecule detection
  • microscopic examination
  • plastic films
  • polymethyl methacrylates
  • thin films
  • fluidic devices

Cite this

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title = "Artificial bilayer lipid membranes (BLMs) on-chip for single molecule sensing",
abstract = "A polymer microfluidic device for the formation of artificial bilayer lipid membranes (BLMs) on-chip is described. The device is fabricated from thin, transparent films of poly(methyl methacrylate), allowing for optical monitoring of the BLM. In addition, detection of single fluorescently-labeled lipid molecules using conventional epifluorescence microscopy is described.",
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Artificial bilayer lipid membranes (BLMs) on-chip for single molecule sensing. / Sandison, Mairi E.; Malleo, Daniele; Holmes, David; Berry, Richard; Morgan, Hywel.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5838, 47, 09.12.2005, p. 252-257.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Artificial bilayer lipid membranes (BLMs) on-chip for single molecule sensing

AU - Sandison, Mairi E.

AU - Malleo, Daniele

AU - Holmes, David

AU - Berry, Richard

AU - Morgan, Hywel

PY - 2005/12/9

Y1 - 2005/12/9

N2 - A polymer microfluidic device for the formation of artificial bilayer lipid membranes (BLMs) on-chip is described. The device is fabricated from thin, transparent films of poly(methyl methacrylate), allowing for optical monitoring of the BLM. In addition, detection of single fluorescently-labeled lipid molecules using conventional epifluorescence microscopy is described.

AB - A polymer microfluidic device for the formation of artificial bilayer lipid membranes (BLMs) on-chip is described. The device is fabricated from thin, transparent films of poly(methyl methacrylate), allowing for optical monitoring of the BLM. In addition, detection of single fluorescently-labeled lipid molecules using conventional epifluorescence microscopy is described.

KW - lipid bilayers

KW - microfluidics

KW - rapid fabrication

KW - single molecule detection

KW - microscopic examination

KW - plastic films

KW - polymethyl methacrylates

KW - thin films

KW - fluidic devices

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U2 - 10.1117/12.608645

DO - 10.1117/12.608645

M3 - Conference article

VL - 5838

SP - 252

EP - 257

JO - Proceedings of SPIE

JF - Proceedings of SPIE

SN - 0277-786X

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ER -