Abstract
The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ∼42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.
| Original language | English |
|---|---|
| Title of host publication | Plasmonics in Biology and Medicine XIII |
| Editors | Tuan Vo-Dinh, Joseph R. Lakowicz, Ho-Pui A. Ho, Krishanu Ray |
| Number of pages | 4 |
| Volume | 9724 |
| DOIs | |
| Publication status | Published - 22 Apr 2016 |
| Event | Plasmonics in Biology and Medicine XIII - Moscone Center, San Francisco, United States Duration: 15 Feb 2016 → 16 Feb 2016 http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016 |
Publication series
| Name | Proceedings of SPIE |
|---|---|
| Publisher | SPIE |
| Volume | 9724 |
| ISSN (Print) | 1605-7422 |
| ISSN (Electronic) | 2410-9045 |
Conference
| Conference | Plasmonics in Biology and Medicine XIII |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 15/02/16 → 16/02/16 |
| Internet address |
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Keywords
- aptamer
- binding assay
- interleukin 6
- plasmonic nanoparticles
- protein detection
- surface enhance Raman spectroscopy (SERS)
- biosensors
- absorbance
- cancer
- dynamic light scattering
- nano silver particles
- optical properties
Cite this
}
Aptamer conjugated silver nanoparticles for the detection of interleukin 6. / Locke, Andrea K.; Norwood, Nicole; Marks, Haley L.; Schechinger, Monika; Jackson, George W.; Graham, Duncan; Coté, Gerard L.
Plasmonics in Biology and Medicine XIII. ed. / Tuan Vo-Dinh; Joseph R. Lakowicz; Ho-Pui A. Ho; Krishanu Ray. Vol. 9724 2016. 972412 (Proceedings of SPIE; Vol. 9724).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book
TY - GEN
T1 - Aptamer conjugated silver nanoparticles for the detection of interleukin 6
AU - Locke, Andrea K.
AU - Norwood, Nicole
AU - Marks, Haley L.
AU - Schechinger, Monika
AU - Jackson, George W.
AU - Graham, Duncan
AU - Coté, Gerard L.
N1 - Locke, A. K., Norwood, N., Marks, H. L., Schechinger, M., Jackson, G. W., Graham, D., & Coté, G. L., "Aptamer conjugated silver nanoparticles for the detection of interleukin 6," Plasmonics in Biology and Medicine XIII, T. Vo-Dinh, J. R. Lakowicz, H-P. A. Ho, & K. Ray (Eds.), Proceedings of SPIE; Vol. 9724, 972412 2016. Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2213896
PY - 2016/4/22
Y1 - 2016/4/22
N2 - The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ∼42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.
AB - The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ∼42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.
KW - aptamer
KW - binding assay
KW - interleukin 6
KW - plasmonic nanoparticles
KW - protein detection
KW - surface enhance Raman spectroscopy (SERS)
KW - biosensors
KW - absorbance
KW - cancer
KW - dynamic light scattering
KW - nano silver particles
KW - optical properties
UR - http://www.scopus.com/inward/record.url?scp=84982131459&partnerID=8YFLogxK
UR - http://proceedings.spiedigitallibrary.org/volume.aspx?conferenceid=3624&volumeid=17548
UR - http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016
U2 - 10.1117/12.2213896
DO - 10.1117/12.2213896
M3 - Conference contribution book
AN - SCOPUS:84982131459
SN - 9781628419580
VL - 9724
T3 - Proceedings of SPIE
BT - Plasmonics in Biology and Medicine XIII
A2 - Vo-Dinh, Tuan
A2 - Lakowicz, Joseph R.
A2 - Ho, Ho-Pui A.
A2 - Ray, Krishanu
ER -