Aptamer conjugated silver nanoparticles for the detection of interleukin 6

Andrea K. Locke, Nicole Norwood, Haley L. Marks, Monika Schechinger, George W. Jackson, Duncan Graham, Gerard L. Coté

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

1 Citation (Scopus)

Abstract

The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ∼42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.

LanguageEnglish
Title of host publicationPlasmonics in Biology and Medicine XIII
EditorsTuan Vo-Dinh, Joseph R. Lakowicz, Ho-Pui A. Ho, Krishanu Ray
Number of pages4
Volume9724
DOIs
Publication statusPublished - 22 Apr 2016
EventPlasmonics in Biology and Medicine XIII - Moscone Center, San Francisco, United States
Duration: 15 Feb 201616 Feb 2016
http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2016

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume9724
ISSN (Print)1605-7422
ISSN (Electronic)2410-9045

Conference

ConferencePlasmonics in Biology and Medicine XIII
CountryUnited States
CitySan Francisco
Period15/02/1616/02/16
Internet address

Fingerprint

interleukins
Silver
Nanoparticles
Interleukin-6
silver
proteins
Proteins
nanoparticles
fever
Metal Nanoparticles
arthritis
Dengue Virus
Dengue
biomarkers
Biosensing Techniques
viruses
Biomarkers
Virus Diseases
Dynamic light scattering
Hydrodynamics

Keywords

  • aptamer
  • binding assay
  • interleukin 6
  • plasmonic nanoparticles
  • protein detection
  • surface enhance Raman spectroscopy (SERS)
  • biosensors
  • absorbance
  • cancer
  • dynamic light scattering
  • nano silver particles
  • optical properties

Cite this

Locke, A. K., Norwood, N., Marks, H. L., Schechinger, M., Jackson, G. W., Graham, D., & Coté, G. L. (2016). Aptamer conjugated silver nanoparticles for the detection of interleukin 6. In T. Vo-Dinh, J. R. Lakowicz, H-P. A. Ho, & K. Ray (Eds.), Plasmonics in Biology and Medicine XIII (Vol. 9724). [972412] (Proceedings of SPIE; Vol. 9724). https://doi.org/10.1117/12.2213896
Locke, Andrea K. ; Norwood, Nicole ; Marks, Haley L. ; Schechinger, Monika ; Jackson, George W. ; Graham, Duncan ; Coté, Gerard L. / Aptamer conjugated silver nanoparticles for the detection of interleukin 6. Plasmonics in Biology and Medicine XIII. editor / Tuan Vo-Dinh ; Joseph R. Lakowicz ; Ho-Pui A. Ho ; Krishanu Ray. Vol. 9724 2016. (Proceedings of SPIE).
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Locke, AK, Norwood, N, Marks, HL, Schechinger, M, Jackson, GW, Graham, D & Coté, GL 2016, Aptamer conjugated silver nanoparticles for the detection of interleukin 6. in T Vo-Dinh, JR Lakowicz, H-PA Ho & K Ray (eds), Plasmonics in Biology and Medicine XIII. vol. 9724, 972412, Proceedings of SPIE, vol. 9724, Plasmonics in Biology and Medicine XIII, San Francisco, United States, 15/02/16. https://doi.org/10.1117/12.2213896

Aptamer conjugated silver nanoparticles for the detection of interleukin 6. / Locke, Andrea K.; Norwood, Nicole; Marks, Haley L.; Schechinger, Monika; Jackson, George W.; Graham, Duncan; Coté, Gerard L.

Plasmonics in Biology and Medicine XIII. ed. / Tuan Vo-Dinh; Joseph R. Lakowicz; Ho-Pui A. Ho; Krishanu Ray. Vol. 9724 2016. 972412 (Proceedings of SPIE; Vol. 9724).

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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T1 - Aptamer conjugated silver nanoparticles for the detection of interleukin 6

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AU - Norwood, Nicole

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N1 - Locke, A. K., Norwood, N., Marks, H. L., Schechinger, M., Jackson, G. W., Graham, D., & Coté, G. L., "Aptamer conjugated silver nanoparticles for the detection of interleukin 6," Plasmonics in Biology and Medicine XIII, T. Vo-Dinh, J. R. Lakowicz, H-P. A. Ho, & K. Ray (Eds.), Proceedings of SPIE; Vol. 9724, 972412 2016. Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2213896

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N2 - The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ∼42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.

AB - The controlled assembly of plasmonic nanoparticles by a molecular binding event has emerged as a simple yet sensitive methodology for protein detection. Metallic nanoparticles (NPs) coated with functionalized aptamers can be utilized as biosensors by monitoring changes in particle optical properties, such as the LSPR shift and enhancement of the SERS spectra, in the presence of a target protein. Herein we test this method using two modified aptamers selected for the protein biomarker interleukin 6, an indicator of the dengue fever virus and other diseases including certain types of cancers, diabetes, and even arthritis. IL6 works by inducing an immunological response within the body that can be either anti-inflammatory or pro-inflammatory. The results show that the average hydrodynamic diameter of the NPs as measured by Dynamic Light Scattering was ∼42 nm. After conjugation of the aptamers, the peak absorbance of the AgNPs shifted from 404 to 408 nm indicating a surface modification of the NPs due to the presence of the aptamer. Lastly, preliminary results were obtained showing an increase in SERS intensity occurs when the IL-6 protein was introduced to the conjugate solution but the assay will still need to be optimized in order for it to be able to monitor varying concentration changes within and across the desired range.

KW - aptamer

KW - binding assay

KW - interleukin 6

KW - plasmonic nanoparticles

KW - protein detection

KW - surface enhance Raman spectroscopy (SERS)

KW - biosensors

KW - absorbance

KW - cancer

KW - dynamic light scattering

KW - nano silver particles

KW - optical properties

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M3 - Conference contribution book

SN - 9781628419580

VL - 9724

T3 - Proceedings of SPIE

BT - Plasmonics in Biology and Medicine XIII

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A2 - Lakowicz, Joseph R.

A2 - Ho, Ho-Pui A.

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ER -

Locke AK, Norwood N, Marks HL, Schechinger M, Jackson GW, Graham D et al. Aptamer conjugated silver nanoparticles for the detection of interleukin 6. In Vo-Dinh T, Lakowicz JR, Ho H-PA, Ray K, editors, Plasmonics in Biology and Medicine XIII. Vol. 9724. 2016. 972412. (Proceedings of SPIE). https://doi.org/10.1117/12.2213896