Abstract
| Language | English |
|---|---|
| Title of host publication | Frontiers and Advances in Molecular Spectroscopy |
| Editors | Jaan Laane |
| Place of Publication | Amsterdam |
| Pages | 77-100 |
| Number of pages | 24 |
| Edition | 1st |
| Publication status | Published - 13 Nov 2017 |
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Keywords
- IR spectroscopy
- DNA
- DNA structure
Cite this
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Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA. / Hithell, Gordon Robert; Ramakers, Lennart A. I.; Burley, Glenn A.; Hunt, Neil T.
Frontiers and Advances in Molecular Spectroscopy. ed. / Jaan Laane. 1st. ed. Amsterdam, 2017. p. 77-100.Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA
AU - Hithell, Gordon Robert
AU - Ramakers, Lennart A. I.
AU - Burley, Glenn A.
AU - Hunt, Neil T.
PY - 2017/11/13
Y1 - 2017/11/13
N2 - Ultrafast two-dimensional infrared (2D-IR) spectroscopy is a powerful probe of the structural and vibrational dynamics of proteins and enzymes in the solution phase. Until recently, relatively few applications of 2D-IR to DNA had been reported, but this is beginning to change rapidly, showing that the vibrational modes of DNA are sensitive reporters of base-pairing and stacking and allowing site-specific probing of the nature of the complex interactions of the DNA macromolecule with its solvent environment. Most recently, 2D-IR spectroscopy has been used to probe the minor-groove ligand binding mechanism and reveal the melting of double stranded DNA in real time, offering the potential for 2D-IR to provide mechanistic insight into the behaviour of this most fundamental of biological molecules in the solution phase. The experimental methods used to obtain 2D-IR spectra are first described along with a discussion of the 2D-IR spectral features relevant to DNA studies before a review of the current state of the art of 2D-IR spectroscopy applications to DNA is presented.
AB - Ultrafast two-dimensional infrared (2D-IR) spectroscopy is a powerful probe of the structural and vibrational dynamics of proteins and enzymes in the solution phase. Until recently, relatively few applications of 2D-IR to DNA had been reported, but this is beginning to change rapidly, showing that the vibrational modes of DNA are sensitive reporters of base-pairing and stacking and allowing site-specific probing of the nature of the complex interactions of the DNA macromolecule with its solvent environment. Most recently, 2D-IR spectroscopy has been used to probe the minor-groove ligand binding mechanism and reveal the melting of double stranded DNA in real time, offering the potential for 2D-IR to provide mechanistic insight into the behaviour of this most fundamental of biological molecules in the solution phase. The experimental methods used to obtain 2D-IR spectra are first described along with a discussion of the 2D-IR spectral features relevant to DNA studies before a review of the current state of the art of 2D-IR spectroscopy applications to DNA is presented.
KW - IR spectroscopy
KW - DNA
KW - DNA structure
UR - https://www.elsevier.com/books/frontiers-and-advances-in-molecular-spectroscopy/laane/978-0-12-811220-5
M3 - Chapter
SN - 9780128112205
SP - 77
EP - 100
BT - Frontiers and Advances in Molecular Spectroscopy
A2 - Laane, Jaan
CY - Amsterdam
ER -