Projects per year
Abstract
Ultrafast two-dimensional infrared (2D-IR) spectroscopy is a powerful probe of the structural and vibrational dynamics of proteins and enzymes in the solution phase. Until recently, relatively few applications of 2D-IR to DNA had been reported, but this is beginning to change rapidly, showing that the vibrational modes of DNA are sensitive reporters of base-pairing and stacking and allowing site-specific probing of the nature of the complex interactions of the DNA macromolecule with its solvent environment. Most recently, 2D-IR spectroscopy has been used to probe the minor-groove ligand binding mechanism and reveal the melting of double stranded DNA in real time, offering the potential for 2D-IR to provide mechanistic insight into the behaviour of this most fundamental of biological molecules in the solution phase. The experimental methods used to obtain 2D-IR spectra are first described along with a discussion of the 2D-IR spectral features relevant to DNA studies before a review of the current state of the art of 2D-IR spectroscopy applications to DNA is presented.
Original language | English |
---|---|
Title of host publication | Frontiers and Advances in Molecular Spectroscopy |
Editors | Jaan Laane |
Place of Publication | Amsterdam |
Publisher | Elsevier Science |
Pages | 77-100 |
Number of pages | 24 |
Edition | 1st |
ISBN (Print) | 9780128112205 |
Publication status | Published - 13 Nov 2017 |
Keywords
- IR spectroscopy
- DNA
- DNA structure
Fingerprint
Dive into the research topics of 'Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA'. Together they form a unique fingerprint.Projects
- 1 Finished
-
EPSRC Doctoral Training Grant - DTA, University of Strathclyde | Hithell, Gordon Robert
Hunt, N. (Principal Investigator), Burley, G. (Co-investigator) & Hithell, G. R. (Research Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/10/13 → 23/02/19
Project: Research Studentship - Internally Allocated