Ultrafast two-dimensional infrared (2D-IR) spectroscopy is a powerful probe of the structural and vibrational dynamics of proteins and enzymes in the solution phase. Until recently, relatively few applications of 2D-IR to DNA had been reported, but this is beginning to change rapidly, showing that the vibrational modes of DNA are sensitive reporters of base-pairing and stacking and allowing site-specific probing of the nature of the complex interactions of the DNA macromolecule with its solvent environment. Most recently, 2D-IR spectroscopy has been used to probe the minor-groove ligand binding mechanism and reveal the melting of double stranded DNA in real time, offering the potential for 2D-IR to provide mechanistic insight into the behaviour of this most fundamental of biological molecules in the solution phase. The experimental methods used to obtain 2D-IR spectra are first described along with a discussion of the 2D-IR spectral features relevant to DNA studies before a review of the current state of the art of 2D-IR spectroscopy applications to DNA is presented.
|Title of host publication||Frontiers and Advances in Molecular Spectroscopy|
|Place of Publication||Amsterdam|
|Number of pages||24|
|Publication status||Published - 13 Nov 2017|
- IR spectroscopy
- DNA structure
Hithell, G. R., Ramakers, L. A. I., Burley, G. A., & Hunt, N. T. (2017). Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA. In J. Laane (Ed.), Frontiers and Advances in Molecular Spectroscopy (1st ed., pp. 77-100). Amsterdam: Elsevier Science.