Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA

Gordon Robert Hithell, Lennart A. I. Ramakers, Glenn A. Burley, Neil T. Hunt

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Ultrafast two-dimensional infrared (2D-IR) spectroscopy is a powerful probe of the structural and vibrational dynamics of proteins and enzymes in the solution phase. Until recently, relatively few applications of 2D-IR to DNA had been reported, but this is beginning to change rapidly, showing that the vibrational modes of DNA are sensitive reporters of base-pairing and stacking and allowing site-specific probing of the nature of the complex interactions of the DNA macromolecule with its solvent environment. Most recently, 2D-IR spectroscopy has been used to probe the minor-groove ligand binding mechanism and reveal the melting of double stranded DNA in real time, offering the potential for 2D-IR to provide mechanistic insight into the behaviour of this most fundamental of biological molecules in the solution phase. The experimental methods used to obtain 2D-IR spectra are first described along with a discussion of the 2D-IR spectral features relevant to DNA studies before a review of the current state of the art of 2D-IR spectroscopy applications to DNA is presented.
LanguageEnglish
Title of host publicationFrontiers and Advances in Molecular Spectroscopy
EditorsJaan Laane
Place of PublicationAmsterdam
Pages77-100
Number of pages24
Edition1st
Publication statusPublished - 13 Nov 2017

Fingerprint

dynamic structural analysis
deoxyribonucleic acid
probes
spectroscopy
macromolecules
grooves
enzymes
vibration mode
melting
proteins
ligands
molecules
interactions

Keywords

  • IR spectroscopy
  • DNA
  • DNA structure

Cite this

Hithell, G. R., Ramakers, L. A. I., Burley, G. A., & Hunt, N. T. (2017). Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA. In J. Laane (Ed.), Frontiers and Advances in Molecular Spectroscopy (1st ed., pp. 77-100). Amsterdam.
Hithell, Gordon Robert ; Ramakers, Lennart A. I. ; Burley, Glenn A. ; Hunt, Neil T. / Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA. Frontiers and Advances in Molecular Spectroscopy. editor / Jaan Laane. 1st. ed. Amsterdam, 2017. pp. 77-100
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Hithell, GR, Ramakers, LAI, Burley, GA & Hunt, NT 2017, Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA. in J Laane (ed.), Frontiers and Advances in Molecular Spectroscopy. 1st edn, Amsterdam, pp. 77-100.

Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA. / Hithell, Gordon Robert; Ramakers, Lennart A. I.; Burley, Glenn A.; Hunt, Neil T.

Frontiers and Advances in Molecular Spectroscopy. ed. / Jaan Laane. 1st. ed. Amsterdam, 2017. p. 77-100.

Research output: Chapter in Book/Report/Conference proceedingChapter

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AB - Ultrafast two-dimensional infrared (2D-IR) spectroscopy is a powerful probe of the structural and vibrational dynamics of proteins and enzymes in the solution phase. Until recently, relatively few applications of 2D-IR to DNA had been reported, but this is beginning to change rapidly, showing that the vibrational modes of DNA are sensitive reporters of base-pairing and stacking and allowing site-specific probing of the nature of the complex interactions of the DNA macromolecule with its solvent environment. Most recently, 2D-IR spectroscopy has been used to probe the minor-groove ligand binding mechanism and reveal the melting of double stranded DNA in real time, offering the potential for 2D-IR to provide mechanistic insight into the behaviour of this most fundamental of biological molecules in the solution phase. The experimental methods used to obtain 2D-IR spectra are first described along with a discussion of the 2D-IR spectral features relevant to DNA studies before a review of the current state of the art of 2D-IR spectroscopy applications to DNA is presented.

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BT - Frontiers and Advances in Molecular Spectroscopy

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Hithell GR, Ramakers LAI, Burley GA, Hunt NT. Applications of 2D-IR spectroscopy to probe the structural dynamics of DNA. In Laane J, editor, Frontiers and Advances in Molecular Spectroscopy. 1st ed. Amsterdam. 2017. p. 77-100