Application of molecularly imprinted polymers in supercritical fluid chromatography

Arndt Ellwanger, Paul K. Owens, Lars Karlsson, Sami Bayoudh, Peter Cormack, David Sherrington, Börje Sellergren

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Molecularly imprinted polymers (MIPs), for the templates free base racemic propranolol and the l-enantiomer of phenylalanine anilide (L-PA), were investigated as stationary phases in supercritical fluid chromatography (SFC). Large retention differences were observed on the propranolol MIP for both the template molecule and the structural analogue metoprolol compared to that observed on the corresponding blank polymer. Mobile phase composition and solute concentration were found to affect this retention behaviour. The phenylalanine anilide MIP (L-PA MIP) was found to be enantioselective in SFC with stronger retention observed for the template enantiomer. Throughout the study, characteristic imprinting peak shapes for the stronger retained template molecule were observed for both MIPs examined. After a number of days under supercritical fluid conditions, the performance of the photochemically initiated L-PA MIP was found to significantly deteriorate whereas the thermally initiated propranolol MIP revealed only small changes in its separation performance after a long term of operation. The separation behaviour of these two MIPs in SFC was compared with results obtained on the same columns in high-performance liquid chromatography (HPLC) both before and after their application in SFC.
Original languageEnglish
Pages (from-to)317-327
Number of pages11
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - 3 Nov 2000


  • chiral stationary phases
  • SFC
  • molecularly imprinted polymers
  • propranolol
  • phenylalanine

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    Ellwanger, A., Owens, P. K., Karlsson, L., Bayoudh, S., Cormack, P., Sherrington, D., & Sellergren, B. (2000). Application of molecularly imprinted polymers in supercritical fluid chromatography. Journal of Chromatography A , 897(1-2), 317-327.